Essay On Effect Of Temperature On Enzyme Activity

805 Words 4 Pages
Abstract The purpose of this investigation is to determine whether the temperature can affect the reaction rate of enzyme activity. The enzyme activity was measured by five different temperatures, separated in tubes which later got measured by their height of foam after 10 minutes. Freshly-prepared potato juice was used as the enzyme for catalase. Since temperature is measured by the kinetic energy of molecules, the higher the temperature, the more movement of molecules. The lowest temperature makes it more likely the molecules will not come in contact with each other.
Introduction
The effect of temperature on enzyme activity was presented by enzyme catalase, found in membrane-bound vesicles called peroxides. Research findings suggest that
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Each test tube had a mark indicating 1 cm and 3 cm. Add potato juice up to the 1cm on every tube. Tube 1 was located in an ice-water bath to a 250mL beaker. Tube 2 was just placed on a test tube rack on your lab bench for room temperature. Test tube 3 was placed in a bath at 50®C. Tube 4 was placed in a water bath at 70®C. Tube 5 was placed inside of a beaker with boiling water on a hot plate; while wearing your goggles. The test tubes where left for 10 minutes unbothered. The thermometer was brought out to measure the temperatures of the room, ice, and boiling water. After 10 more minutes all tubes where collected and hydrogen peroxide got added to the 3cm line. Gently shaking each tube to mix the ingredients together. After 30 seconds or until each tube stops foaming use a ruler and measure the amount of foam produced in each tube. The foam is produced when catalase is in contact with hydrogen peroxide. Tube 1 with Temperature 2®C was measured at the highest foam height of 30mm. Tube 2 with the temperature at 23®C measured 23mm in foam height. Tube 3 with the temperature of 50®C foam height reached 2mm. But for tube 4 which was temperature 70®C and tube 5 that was temperature 100®C foam height ended up as 0mm, because if above 40®C most enzymes activities in living tissue become denatured meaning their secondary or tertiary protein structure breaks …show more content…
Again, there was not a difference in tubes four and five in foam height. My conclusion is that when you have hydrogen periodic and potato juice the lower the temperature, the higher the catalase reaction will be. Because test tube two was not far behind test tube one when it came to foam height, this study raises the question of whether there might be an optimum temperature in enzyme activity. On the basis of the results of this study, the hypothesis presented can be taken in to account, and be accepted. Additional experimentation with different temperatures is suggested. Also, the requirement of enzyme activity on temperature has been labeled with two consisting processes: the catalytic reaction and the irreversible inactivation ( Biochem

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