Enzyme Lab

Improved Essays
Abstract

This experiment observed the effects of enzyme’s concentration on the enzyme’s activity. This was determined by measuring the rate of the reaction under different concentration, and see whether the reaction increases when the concentration high or not. The consequences of the experiment indicated that the rate of the reaction of enzyme increases whenever the concentration is high. Also, if the concentration is low, the reaction rate will be low too.

Introduction

Enzymes are proteins that make the chemical reactions faster by reducing the activation energy that is required to start the reaction, and they also act as catalyst. The substrate, which the enzyme acts upon, binds to the part of enzyme that is known as active site.
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Then specific test materials were added to each of them as following on the table 1. After preparing all the test tubes, and the concentration of enzyme, two spot plates were put next to each other to form 4 rows that are labeled from A-D, and 6 columns that are labeled from 1 to 6. After that, five drops of starch, and two drops of buffer were placed to all rows and columns in the spot plates. As quickly as possible, one drop of undulated enzyme and test tube 1 were added to row A, one drop of test tube 6 was added into row B, one drop of test tube 7 was added into raw C, and lastly one drop of test tube 8 was added into raw D. Afterwards, directly add one drop of iodine to column 1 and observe the results after 5 minutes, then add another drop of iodine to column 2, and wait another 5 minutes. Continue to add the iodine to all columns with 5 minutes pause. The observations had been recorded by writing 1 when the color turned blue-black, and 2 when the color didn’t change and remained light …show more content…
Results

The rate of reaction depends and controlled by the enzyme concentration. The more enzyme concentration on the reaction, the faster the reaction will be. The reason is that there will be more enzymes present in the active site, which will bind to the substrates. According to that, the reaction rate will increase. The results on table 2 indicate that as times go on, the more concentrated enzymes will turn brown. Also, it indicates that the first row, which is undiluted higher concentration of enzymes, got the results earlier than the rest, which proves the hypothesis that being tested.

Enzyme/
Test tube
Time
0
Time
5
Time
10
Time
15 Time
20
Time 25

1
1
2
2
2
2
2

6
1
1
2
2
2
2

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