Enzyme Reaction Experiment

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The effect of temperature on the rate of reaction on Enzyme reaction


This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalase. The biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed. Enzymes are globular proteins folded into a complex 3-dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically. Enzymes speed up reactions as they lower the activation energy. In this experiment, the enzyme used is catalase; which is found in both plant cells and animal cells. The catalase I will use is yeast which has 37oC optimal temperature similar to the
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1 cm 3 of water is added to the test tube is marked; then the distance between the two markings are measured which represents 1cm3. The test tube is mark at the same distance, the test tube is marked to the appropriate number. 1cm 3 of yeast cell suspension is placed into the marked test tube using a pipet which is emptied from the water. 2cm 3 of hydrogen peroxide solution is placed into another test tube using a pipette. Both test tubes are placed in a water bath test tube rack which is maintained at a specific temperature; the test tubes equilibrate for 3 minutes. After the 3 minutes both test tubes are removed from the water bath and the hydrogen peroxide is poured into the calibrated test tube and mixed to avoid yeast suspension residue. It is timed for 30 seconds using a stopwatch.
The volume produced after 30 seconds is noted. This is repeated to all temperatures over the range of water baths

My Results

Temperature (oC)

Water bath Temperatures (oC) 0 25 30 35 40 45 50 55
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Only when hydrogen peroxide is available, the catalase reaction can take place which is demonstrated in the results. In fact, the catalase reaction is dependent on the substrate concentration. If you have an excess of enzyme but not enough substrate, the reaction will be limited by the substrate availability. Once you add more hydrogen peroxide to the solution, the reaction rate will increase as more substrate molecules can collide with the enzyme, forming more product. The result is an increasing amount of bubbles produced in the test tube with the increase in

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