Creatinine Clearance Lab Report

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This lab included various experiments that demonstrated how renal system responses and reacts when subjects consuming different types of fluids. Respective fluid volumes are based on each subject’s body mass. Based on each subject’s body mass, after converted from pounds to kilograms, every subjects has different volume and fluids for ingestion. For the control subject, she ingested no fluid throughout entire lab which represented the conditions of normal fluid. For the hypotonic subjects, she ingested 854mL of water within 10 minutes which represented the dilution of bodily fluids by seeing the urine became clear and clear each time. For isotonic subjects, who consumed 1120mL of isotonic saline (0.9% NaCl), shown the importance of sodium related …show more content…
So, creatinine clearance needs to be calculated in order to estimate GFR. Since clearance defined as the volume of plasma that is completely cleared or cleaned by the kidney when excreting substance in urine, it is a good way to determine if the kidneys are functioning properly. Creatinine is a metabolic byproduct of skeletal muscle that is freely filtered, not re-absorbed and with little secretion. Although creatinine clearance is not completely accurate when measuring GFR, it provides better and closer approximation than insulin clearance. GFR is the volume of filtrate formed per unit time. By comparing the clearance of creatinine to GFR, it was able to determine whether there was any reabsorption or secretion of creatinine by the kidney. If a substance is filtered at the glomerulus and fully, completely reabsorbed and not secreted, the clearance will be zero. If a substance is filtered and unmodified by the nephron, clearance will equal GFR. Clearance will be greater than GFR when a substance is filtered and secreted, but not reabsorbed (Sherwood, …show more content…
Research by Horswill, Murray, Stofan, Stover, and Zachwieja showed that specific gravity is strongly correlated with urine osmolality (2006). Values greater than 1.020 indicate dehydration and lower than 1.010 indicate over hydration. The control subject has an increase in specific gravity values over time due to no ingestion of fluids, and thus leading to a slightly increase in concentrations of solutes in urine. Similarly, alkalosis subject’s specific gravity increased because of excess amounts of sodium in the urine as bicarbonate was reabsorbed. This shows that there was an increase in concentration of solutes in urine. Specific gravity of the hypotonic subject decreased due to an excess intake of water diluting the urine. Isotonic subject’s specific gravity stayed constant because the composition of the saline solution was similar to the body’s

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