Busulfan Case Study

831 Words 4 Pages
Busulfan is anticancer drug caused variety of adverse effects for cancer patients. It’s used to treatment of chronic leukaemia but it could cause damage to the male reproductive system as one of its adverse effects. This study aim to investigate the effect of L-carnitine and L-arginine on semen quality, oxidative stress parameters, caspase3 and testes cell energy after busulfan treatment.
Materials and Methods: thirty-two adult male rats were divided into four groups. Group I (Control) received a single dose (I.P.) of DMSO and distilled water (1:1). Group II (Bus) received a single of busulfan (20 mg/kg) (I.P.). Group III (Bus+L-arg) received busulfan plus 1mL of L-arginine daily by oral gavages. Group IV (Bus+L-car) received a single dose
…show more content…
As an alkylating agent, it works by sticking to one of the cancer cell's DNA strands; thus, the cancer cells undergo apoptosis (2). Busulfan is used for the chemotherapeutic drug of leukemia, as it is relatively cheap drug. As through, after treatment, busulfan caused adverse effects in various organs, such as the reproductive tract organs. Busulfan treatment induces oligospermia and even azoospermia and testicular atrophy in young male patients, leading to sterility in some cases (3).
L-carnitine is concentrated in high energy demanding tissues like muscles, skeletal and cardiac and in particular in reproductive system, the epididymis. L-carnitine was first isolated from beef muscle in 1905. Approximately 75% of L-carnitine of the body stores is derived from the diet, where as only 25% is synthesized de novo from lysine and
…show more content…
It is a small water-soluble particle important in fat metabolism (4). It plays an important role in long-chain fatty acids oxidation in mitochondria then, producing energy. In addition, adjustment of acyl-CoA / CoA ratio, store energy as acetylcarnitine, and revising the toxic effects of poorly metabolized acyl groups by releasing them as carnitine esters (5). L-carnitine concentration in epididymal fluid and sperms range from 2 upto 100 mmol, i.e. about 2000 fold greater than circulating levels (10-50 mol). Free L-carnitine is taken up from the blood plasma and is transported into the epididymal plasma. It is then diffused passively into the spermatozoa, where it stacks as free and acetylated L-carnitine. The initiation of sperm motility is proportional to concentration of L-carnitine in the epididymal lumen (6). Another potential use of seminal free L-carnitine is in the diagnosis of obstructive azoospermia in the cases of post epididymal obstruction, such as those with vas deference agenesis, have extremely low concentrations of carnitine(7). It is known that L-carnitine has antioxidant, anti-inflammatory and antiapoptotic effects on various pathophysiological conditions

Related Documents