Busulfan is anticancer drug caused variety of adverse effects for cancer patients. It’s used to treatment of chronic leukaemia but it could cause damage to the male reproductive system as one of its adverse effects. This study aim to investigate the effect of L-carnitine and L-arginine on semen quality, oxidative stress parameters, caspase3 and testes cell energy after busulfan treatment.
Materials and Methods: thirty-two adult male rats were divided into four groups. Group I (Control) received a single dose (I.P.) of DMSO and distilled water (1:1). Group II (Bus) received a single of busulfan (20 mg/kg) (I.P.). Group III (Bus+L-arg) received busulfan plus 1mL of L-arginine daily by oral gavages. Group IV (Bus+L-car) received a single dose …show more content…
It is a small water-soluble particle important in fat metabolism (4). It plays an important role in long-chain fatty acids oxidation in mitochondria then, producing energy. In addition, adjustment of acyl-CoA / CoA ratio, store energy as acetylcarnitine, and revising the toxic effects of poorly metabolized acyl groups by releasing them as carnitine esters (5). L-carnitine concentration in epididymal fluid and sperms range from 2 upto 100 mmol, i.e. about 2000 fold greater than circulating levels (10-50 mol). Free L-carnitine is taken up from the blood plasma and is transported into the epididymal plasma. It is then diffused passively into the spermatozoa, where it stacks as free and acetylated L-carnitine. The initiation of sperm motility is proportional to concentration of L-carnitine in the epididymal lumen (6). Another potential use of seminal free L-carnitine is in the diagnosis of obstructive azoospermia in the cases of post epididymal obstruction, such as those with vas deference agenesis, have extremely low concentrations of carnitine(7). It is known that L-carnitine has antioxidant, anti-inflammatory and antiapoptotic effects on various pathophysiological conditions
Materials and Methods: thirty-two adult male rats were divided into four groups. Group I (Control) received a single dose (I.P.) of DMSO and distilled water (1:1). Group II (Bus) received a single of busulfan (20 mg/kg) (I.P.). Group III (Bus+L-arg) received busulfan plus 1mL of L-arginine daily by oral gavages. Group IV (Bus+L-car) received a single dose …show more content…
It is a small water-soluble particle important in fat metabolism (4). It plays an important role in long-chain fatty acids oxidation in mitochondria then, producing energy. In addition, adjustment of acyl-CoA / CoA ratio, store energy as acetylcarnitine, and revising the toxic effects of poorly metabolized acyl groups by releasing them as carnitine esters (5). L-carnitine concentration in epididymal fluid and sperms range from 2 upto 100 mmol, i.e. about 2000 fold greater than circulating levels (10-50 mol). Free L-carnitine is taken up from the blood plasma and is transported into the epididymal plasma. It is then diffused passively into the spermatozoa, where it stacks as free and acetylated L-carnitine. The initiation of sperm motility is proportional to concentration of L-carnitine in the epididymal lumen (6). Another potential use of seminal free L-carnitine is in the diagnosis of obstructive azoospermia in the cases of post epididymal obstruction, such as those with vas deference agenesis, have extremely low concentrations of carnitine(7). It is known that L-carnitine has antioxidant, anti-inflammatory and antiapoptotic effects on various pathophysiological conditions