Beet Cells Lab Report Essay

1302 Words Nov 5th, 2010 6 Pages
The Effect of Temperature on Beet Cell Membranes and Concentration of Betacyanin Released

Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell et al., 2008). Each membrane layer is composed of a phospholipid layer, which is semi-permeable and possesses the ability to control the movement of diffusion. Within the centre of a cell, the vacuole that is responsible for storage and the maintenance of osmotic balance can be located. In this study, we are using beets to study these cellular structures. Beet is a plant whose cells contain a characteristic red pigment, called betacyanin. It is located in the vacuole, which is
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Materials and methods Seven discs of 5mm beet root were cut with a razor blade and rinsed with tap water in a plastic beaker for 10 minutes. Each beet disc was then submersed in a beaker of water with temperature of either –5°C, 4°C, 42°C, 55°C, 70°C and room temperature for precisely 2 minutes. The exact time of extraction was recorded when the discs were placed into large test tube with white caps (labelled with temperatures) immediately after the water bath. The test tubes were then rested on the bench for a total of 10 minutes and were vortexed at 5 minutes and 10 minutes. After 10 minutes, 5 ml of solution from the large test tubes was pipetted into small test tubes and each small test tube was then inserted into the spectrophotometer for measurements of absorbance. A detailed protocol can be found in the Biology 107 Laboratory Manual (2010-2011).

Results In our experiment, we observed that the samples in temperature –5°C and 70°C contained the highest concentration of betacyanin (Figure 2). To obtain these results, we used the standard curve for betacyanin (Figure 1) to figure out the concentration, since the value of betacyanin absorbance could be measured using the spectrophotometer.
From the graph (Figure 2), between the temperature of 4°C, 42°C, 55°C, it was observed that the ranges of concentration was narrow, which shows the increase concentration was quite steady. Generally, an increase of temperature would cause an increase in absorbance of

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