Autoclaved Sucrose Research Paper

It was often demonstrated that autoclaved sucrose was better for growth than filter sterilized sucrose. Autoclaving seems to hydrolyze sucrose into more efficiently utilizable sugars such as fructose. Sucrose was reported to work as a morphogenetic trigger in the establishment of auxiliary buds and branching of adventitious roots [8]. It was found that supplements of sugar cane molasses, banana extract and coconut water to basal media can be a safe option for reducing medium costs. These substrates in addition to sugars, they are sources of vitamins and inorganic ions required growth [9, 10]. 2.4. Vitamins and Myo-inositol Some plants are able to synthesize the essential requirements of vitamins for their development. Some vitamins are required …show more content…
Thiamin is used at concentrations ranging from 0.1 to 10 mg. l-1. Nicotinic acid and pyridoxine, however not essential for cell growth of many species, they are often added to Plant Tissue Culture Media 31 culture media [12]. Nicotinic acid is used in a concentration range 0.1-5 mg. L-1 and pyridoxine is used at 0.1-10 mg. L-1. Other vitamins such as biotin, folic acid, ascorbic acid, pantothenic acid, vitamin E (vitamin E), riboflavin, p-amino-benzoic acid are used in some cell culture media however, they are not growth limiting factors. It was recommended that vitamins should be added to culture media only when the absorption of thiamin is below the desired level or when the cells are postulated to be made at low population densities [14]. Although it is not a vitamin, but a carbohydrate, Myo-inositol is added in low quantities to stimulate cell growth of most plant species [13]. Myo-inositol is believed to play a role in cell division because of its breakdown to ascorbic acid and pectin and incorporation into phosphoinositides and Phosphatidyl-inositol. It is generally used in plant cell and tissue culture media at concentrations of 50-5000 milligram. (Elshahed