Determining the impact of Auxin in pea plants, and its effect on lateral bud growth
Megan Posey
Bio 121-104
John Barry
10/23/16
Abstract Auxin is a hormone that helps to regulate bud growth, cell elongation, and many other aspects of plants. Apical dominance is a phenomenon that occurs due to the presence of auxin and stops lateral bud growth. In this experiment, we tested the hypothesis that a group of pea plants without treatment of auxin would have the most lateral bud growth, the 500 ppm group treated with auxin would have little bud growth, and the 5,000 ppm group treated with auxin wouldn’t have any growth. The significance of these numbers was then determined by the use of a Wilcoxon test. The control group showed to be significantly …show more content…
Each lab table was responsible for growing a pea plant. Nine pea plants were planted in total. The pea plants were separated into three groups. The first group was without any auxin, the second group had 500 ppm of auxin, and the last group had 5,000 ppm of auxin. The growth of the pea plants were recorded and ranked using a Wilcoxon test to determine which peas showed significant growth. The growth of the buds was recorded and put in graphs. Each group obtained a pot from the instructor. After obtaining a pot, labels were applied and written with a ball point pen. Each label had the time and written on it. A small section of folded paper towels was inserted over the holes in the bottom of the pot. The pots were then filled ¾ full of dry vermiculite. Water was then applied to the vermiculite. Four shallow indentations were made inside each pot. Four pea plants were planted, evenly spaced out inside each pot. The plants were then placed in a dark planting flat to …show more content…
It appeared that the control group’s length was significantly longer than the 500 ppm treatments, which showed little growth. The lengths of the buds were ranked and then averages were taken. The lengths were measured in millimeters, and started with 23 mm in the 500 ppm treatment group, growing slowly to 78 mm. The second group was treated with 5,000 ppm of lanolin and then compared to the control treatment. Averages were taken and the buds were ranked by growth. The 5,000 ppm appeared to have no lateral bud growth and therefore all ranked equally. The last table shows the relationship between the 500 ppm and 5,000 ppm treatments of auxin.
It appeared that the bud with the 500 pm showed significantly more lateral bud growth compared to the 5,000 ppm treatment of lanolin The average of the bud growth was recorded in each table and ranks recorded. To determine if there was statistically significant between the control and 500 ppm, the control and 5,000 ppm, and between 500 and 5,000. The Wilcoxon test was taken and calculated using the values in table 4. The equations below were used to calculate significance. The calculations show that there was significant differences between all of the
Megan Posey
Bio 121-104
John Barry
10/23/16
Abstract Auxin is a hormone that helps to regulate bud growth, cell elongation, and many other aspects of plants. Apical dominance is a phenomenon that occurs due to the presence of auxin and stops lateral bud growth. In this experiment, we tested the hypothesis that a group of pea plants without treatment of auxin would have the most lateral bud growth, the 500 ppm group treated with auxin would have little bud growth, and the 5,000 ppm group treated with auxin wouldn’t have any growth. The significance of these numbers was then determined by the use of a Wilcoxon test. The control group showed to be significantly …show more content…
Each lab table was responsible for growing a pea plant. Nine pea plants were planted in total. The pea plants were separated into three groups. The first group was without any auxin, the second group had 500 ppm of auxin, and the last group had 5,000 ppm of auxin. The growth of the pea plants were recorded and ranked using a Wilcoxon test to determine which peas showed significant growth. The growth of the buds was recorded and put in graphs. Each group obtained a pot from the instructor. After obtaining a pot, labels were applied and written with a ball point pen. Each label had the time and written on it. A small section of folded paper towels was inserted over the holes in the bottom of the pot. The pots were then filled ¾ full of dry vermiculite. Water was then applied to the vermiculite. Four shallow indentations were made inside each pot. Four pea plants were planted, evenly spaced out inside each pot. The plants were then placed in a dark planting flat to …show more content…
It appeared that the control group’s length was significantly longer than the 500 ppm treatments, which showed little growth. The lengths of the buds were ranked and then averages were taken. The lengths were measured in millimeters, and started with 23 mm in the 500 ppm treatment group, growing slowly to 78 mm. The second group was treated with 5,000 ppm of lanolin and then compared to the control treatment. Averages were taken and the buds were ranked by growth. The 5,000 ppm appeared to have no lateral bud growth and therefore all ranked equally. The last table shows the relationship between the 500 ppm and 5,000 ppm treatments of auxin.
It appeared that the bud with the 500 pm showed significantly more lateral bud growth compared to the 5,000 ppm treatment of lanolin The average of the bud growth was recorded in each table and ranks recorded. To determine if there was statistically significant between the control and 500 ppm, the control and 5,000 ppm, and between 500 and 5,000. The Wilcoxon test was taken and calculated using the values in table 4. The equations below were used to calculate significance. The calculations show that there was significant differences between all of the