Determination Of The Greatest Amount Of Ascorbic Acid Experiment

Improved Essays
periment 1: Determination of Vitamin C

Name: Mariam Eskandari
Student #: 140856340
Date Conducted: September 8th, 2014
Date Submitted: September 15th, 2014
Partner’s Name: Jessica Macinnis
Lab Section: Monday 5:30, L2
IAs Name: Ryan Hiebert ABSTRACT

This experiment was conducted to calculate the greatest amount of ascorbic acid throughout a selection of 6 different orange juice samples with the help of individual and class data, since some juices seem to have a higher amount of vitamin C than others. The ascorbic acid was measured through the process of titration, using 10 drops of starch indicator, and approximately 0.5g of oxalic acid to determine the amount of vitamin C present with the initial 50 mL iodine solution in the burette.
…show more content…
Titration helped determine the ascertain amount of ascorbic acid in the orange juice by measuring the known volume in a burette and concentration of the iodine solution, with the help of the starch indicator. The class data had a mean of 9.12 and an average standard deviation of 2.22; standard deviations were from 0.63, 0.79, 1.24, 1.64, 1.70, 2.20. The average masses of ascorbic throughout the 6 different oranges juices in order of most to least was 9.92 mg, 9.90 mg, 9.12 mg, 8.19 mg, 6.21 mg, 6.17 mg. According to the data, the best source of orange juice is the Tropicana, since it has the highest average mass of ascorbic acid, therefore, it has the most vitamin C in comparison to the other orange juice samples, despite the fact that the navel oranges should have had the most amount of ascorbic acid since it would have been raw ascorbic acid, and not …show more content…
Fall 2014. Determination of Vitamin C. Pages 28-29 in Chemistry 110 Lab Manual. Wilfrid Laurier University, ON, Canada.

RESULTS

Table 1: Qualitative Results For the 6 Types of Orange Juice to Determine Amount of Vitamin C

Simply Orange Tropicana President’s Choice Frozen Navel Oranges President’s Choice Minute Maid
Qualitative Observations Average orange color, pulp free, solid settlement at bottom of container, Diluted orange color, pulp free, solid settlement at bottom of container, opaque Brighter yellow/ orange color, pulp free, solid settlement at bottom of container Lighter orange color, opaque, pulp free, no solid settlement at bottom of container Brighter yellow/ orange color, no solid settlement, pulp free Vibrant Yellow/Orange, opaque, top layer is brighter than the rest of the sample

Table 2: Quantitative Burette Readings Results for Titration of President’s Choice 100% Frozen Orange Juice with Iodine

Trial 1 Trial 2 Trial 3
Volume of Orange Juice (mL) 25.00 mL 25.00 mL 25.00 mL
Final burette reading (mL) 15.30 mL 26.85 mL 38.8 mL
Initial burette reading (mL) 0.00 mL 15.30 mL 26.85 mL
Volume of iodine used (mL) 15.30 mL 11.55 mL 11.95

Related Documents

  • Improved Essays

    Avocado Research Paper

    • 630 Words
    • 3 Pages

    The rich antioxidants present in avocados are useful in preventing the symptoms of aging. Formation of a healthy nervous system, slowing down of aging process and enhancing the immune system is carried out by glutathione present in the Avocado. Nutrient absorption is enhanced Nutrients are absorbed very easily with the intake of Avocado. In a research study, it is revealed that people who ate avocados in the salad could absorb more of carotenoids than those who did not eat avocados.…

    • 630 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    From what is known regarding ascorbic acid, researchers were able to validate the urgency for the vitamin. For one, it is vital for the production of collagen, a protein found in connective tissues that bind and support other tissues. Lack of collagen explains some of the early symptoms of scurvy. Although scurvy was mainly an issue during the age of exploration, it is still present in modern day life. Without the proper intake of ascorbic acid daily, humans still run the risk of developing this disease.…

    • 1224 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Vitamin C, is found, with iron, calcium, and magnesium also. Its bright color also provides many antioxidants.…

    • 2074 Words
    • 9 Pages
    Improved Essays
  • Improved Essays

    M & M Research Paper

    • 443 Words
    • 2 Pages

    Then fill six new centrifuge tube with the orange juice. Label three of them C1, C2, C3 those three will be the control and the other three E1, E2, E3 these will be the orange juice that will be put through pasteurization. Always do attest three for each so incase if one orange was messed up in some way it won’t play a huge impact on your data. Then place the E1, E2, E3 tubes into the water bath at the required 80 degrees Celsius to pasteurize for 20 minutes. After the 20mintues are up remove the tube from the water bath and allow them to cool off.…

    • 443 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Nutrition Lab Report

    • 1661 Words
    • 7 Pages

    (Able to dissolve in water ) Vitamin C : Required for structure and function of connective tissue and important for repairing as has anti oxidant properties Vitamin B: Categorised into 12 different Vitamins See Below the Main B Vitimins Vitamin B1 : thiamine > The release of Energy from Carbohydrates Vitamin B9 Folic Acid >Important during Pregnancy The following Foods contains a rich source of each of the vitamins identified. Fat Soluble (Able to dissolve in water ) Vitamin A : Beef Liver and…

    • 1661 Words
    • 7 Pages
    Improved Essays
  • Improved Essays

    Black Forest includes all this Vitamin C without sacrificing any taste and actually adding a juicy center containing real fruit juice. Vitamin C is an essential part of the human diet, needed for muscle growth and regeneration, and strengthening the immune system. All these benefits while enjoying a juicy fruit snack--you can’t find that in Tree Top fruit snacks--you’ll need Black Forest Fruit Medley fruit snacks for those superior benefits and taste. The argument can be made that “Many other foods you will eat have Vitamin C”, yes this is true but who really wants to eat vegetables over a fruit snack?…

    • 643 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    In wine tasting, the term “acidity” refers to the fresh, tart and sour attributes of the wine which are evaluated in relation to how well the acidity balances out the sweetness and bitter components of the wine such as tannins. Three primary acids are found in wine grapes: tartaric, malic and citric acids. During the course of winemaking and in the finished wines, acetic, butyric,lactic and succinic acids can play significant roles. Most of the acids involved with wine are fixed acids with the notable exception of acetic acid, mostly found in vinegar, which is volatile and can contribute to the wine fault known asvolatile acidity. Sometimes, additional acids, such as ascorbic, sorbic and sulfurous acids, are used in…

    • 1034 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of…

    • 753 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    This process results in the release of oligo- and polysaccharides from the apple pomace, causing high colloid concentration in the extraction juice43. Some of oligosaccharides mentioned above were studied for their beneficial physiologic effects, such as potential prebiotic activities38, as potential chemoprevention agent or anti-tumor agent by inhibiting the cellular viability of human colon carcinoma cells40, and enhancing fecal excretion of steroids in rat models44. However, some studies did not report the detailed structures of the beneficial oligosaccharides responsible for revealed health benefits while others studied the oligosaccharides structures in the extraction juice43. There is no detailed report on the structural determination of free oligosaccharides with potential benefits in apple…

    • 930 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    In addition, some juice may remain in the pomace of the fruit(apple), leading to incomplete extraction process. Therefore, pectinase is needed to assist juice extraction and separation of undesirable precipitate using centrifugation or sedimentation. Research suggests that the juice yield increases with the concentration of pectin enzymes. The use of pectinase is also critical in clarification process of juices while retaining the nutrients and flavor in it. Some fruits, such as banana, have high pectin (fibre) content that may interfere with the clarification process of the juice.…

    • 964 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    It should be mentioned that the apples pieces, unlike the carrots, were exhibiting browning effects changing their color during the cutting and peeling session and before placing them into the solutions. Also, an important observation that some of the solutions took long time to fully dissolve, for instance, the sugar > 6 hours prior the experiment, and this was not the case in the salt. 3.2. Moisture content In this experiment, two setting were conducted to see the osmotic agents and temperatures effect.…

    • 1013 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Aloe Vera Case Study

    • 898 Words
    • 4 Pages

    Total soluble solids concentration (TSS) was determined using hand refractometer (PAL-3, ATAGO, Japan) according to AOAC, (2000). . 6.1.4.Texture analysis Fruits firmness was determined using a texture analyzer as described by Licodiedoff et al (2016) 6.1.5.…

    • 898 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    However, in excess, it may make the fruits unpalatable even if other components are optimum. In guava, Kumar et al. (1996) reported that increasing levels of nitrogen significantly reduced the fruit acidity as compared to control and the juice acidity decreased appreciably from 0.35 to 0.20% at nitrogen 100 g/tree and least value being at nitrogen level 800 g/tree/year. In mango, the minimum fruit acidity was observed when NPK was applied @ 1000, 500 and 500 g/tree (Gautam et al.,…

    • 1823 Words
    • 8 Pages
    Improved Essays
  • Improved Essays

    Some physical and chemical properties of watermelon rind and sharlyn melon peel powders Proximate composition WAC, OAC and antioxidant activity of watermelon rind and sharlyn melon peel powders Data in Table 2 showed that the watermelon rind powder had higher moisture, ash, fat, protein and carbohydrates (10.61%, 13.09%, 2.44%, 11.17% and 56.02%, respectively) as compared to sharlyn melon peel powder. On the other hand, the sharlyn melon peels had higher content of crud fiber (29.59%) as compared to watermelon rinds (17.28%). The crud fiber content in watermelon rind and sharlyn melon peel powder was higher than navel orange peels (13.38%) and mandarin peels (7.14%) as mentioned by Magda et al. (2008).…

    • 933 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    Vitamin A can be found in milk, cheese, eggs and carrots. All of the vitamin B’s help metabolism, produces energy, and creates a healthy circulatory and nervous system. Sources of vitamins B is meat, fish, eggs, milk and cheese. Vitamin C contributes towards health skin, tissue and muscles Vitamin C can be found in fruits, and green vegetables. Finally, Vitamin D helps the body absorb calcium, and helps bone and tooth formation.…

    • 1939 Words
    • 8 Pages
    Great Essays