M & M Research Paper

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M & M
Three oranges were cut in half and then placed over the juicer. The oranges were then pressed down, while being twisted allowing the juices from the oranges to be collected in the tray below. The orange juice in the trays were then poured into a 250ml beaker with cheese cloth on top of it. The cheesecloth caught the larger pieces of pulp from the oranges while allowing the juice to pass. The orange juice was then transferred into six 1.5ml centrifuge tubes. The six centrifuge were then placed into the centrifuge for two minutes at maximum speed. This step is important due to the fact the orange juice still has pulp in it even though strained though the cheese cloth. After the two minutes are up a pellet should of form on the bottom of each tube. Carefully remove the orange juice without collecting the pellet into a 250ml
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Then fill six new centrifuge tube with the orange juice. Label three of them C1, C2, C3 those three will be the control and the other three E1, E2, E3 these will be the orange juice that will be put through pasteurization. Always do attest three for each so incase if one orange was messed up in some way it won’t play a huge impact on your data. Then place the E1, E2, E3 tubes into the water bath at the required 80 degrees Celsius to pasteurize for 20 minutes. After the 20mintues are up remove the tube from the water bath and allow them to cool off. After transfer the c1, c2, c3, into three cuvettes. Along with transferring E1, E2, E3 into three spate cuvettes. Place C1 though c3 one at a time though the spectrometer. Record each data. Then repeat the process for E1 though E3. Record the data also. If the spectrometer cannot read your control or experiment tubes then a dilution factor must be used. In this experiment the spectrometer could read all six test so no dilution factor

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