A Experiment On Chromatography Essay

825 Words Oct 30th, 2015 4 Pages
In many cases in industry, it is necessary to separate a mixture or solution. In environmental testing, they look for PCB’s in oil, and pesticides in ground water. In the food industry, they can test additives to determine concentrations of preservatives, vitamins, proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks.
Understanding the history of chromatography can help us understand different uses of the technique. A Russian botanist invented chromatography to separate and distinguish plant pigment. Mikhail Semenovich Tsvett trickled a mixture of dissolved pigments through a glass tube filled with a tight calcium carbonate powder that absorbed the pigments to different degrees as they flowed through the tube. This created bands of colors, referred to as a chromatogram. This method was absorption chromatography, and is similar to column chromatography. Tsvett published a quick forgotten paper on this technique, which went forgotten until German chemist Richard Martin Willstatter studied chlorophyll (a type of pigment). Willstatter used…

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