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18 Cards in this Set
- Front
- Back
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what is the solvent used for proton nuclear magnetic resonance ? |
TMS , tetramethylsilane |
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formula for TMS? |
Si (CH3)4 |
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what is spin spin coupling? |
the interference of magnetic fields due to spinning of the proton in the nuclei and the protons next to it |
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what is the solvent for carbon 13 ? and what is the ppm? |
CDCl3, 80 ppm |
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how does mass spectrometry work? |
first you vaporise the gas and then you bombard it with electrons to form the M+ , break covalent bond to fragment the molecule |
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ratio of peaks of two Cl and two Br? |
two Cl 9:6:1 two Br 1:2:1 |
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GLC linked with mass spectrometer can be used for what? |
forensics environmental monitoring of pollutants drug testing
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what is the stationary phase in TLC? |
Al2O3 or SiO2 they are polar |
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in GLC how are mixtures identified? and what does the area under the peak tell you? |
identified using the retention time and the area represents the concentration. |
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uses of HPLC? |
medical research to separate peptides and proteins analyse urine samples of athletes for banned substances monitoring pollutants,food standard agencies to check accuracy of food labels |
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what is the mobile and stationary phase for HPLC? |
stationary phase is the non volatile liquid ex.silica mobile phase is a polar solvent like water |
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GLC uses? |
test fuel for racing test steroid in athletes medical diagnosis of blood sample |
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difference between adsorption and partition chromatography? |
PC= separation due to different solubilities in mobile and stationary phase, ex. paper chromatography and gas liquid chromatography AC= separation due to molecules having different attraction between the compounds,stationary phase, relative to their solubility in solvent ex. thin layer chromatography |
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what is Rf value? |
the distance moved by the compound or solute from the baseline over the distance travelled by the solvent front |
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why does non competitive inhibitor enzyme reduce the rate of reaction |
the inhibitor reduces the number of working active sites, less active site is available |
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for HPLC, give an example of a stationary phase and a mobile phase |
stationary phase= silica(non volatile liquid) mobile= water/methanol mobile phase usually polar |
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what is the ppm for TMS? |
0ppm |
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what is the stationary phase for TLC? |
alumina and silica |
