The purpose of this experiment was to determine the working concentration of Tetracycline (TNC) on Escherichia coli (EC). We used EC because of its characteristics of fast growth in different environments, complete gene sequence, and availability. Our antibiotic, TNC, is a broad spectrum antibiotic which is effective to many types of bacteria. This is because it obstructs tRNA anticodon from reading the mRNA codons in the 30S ribosomal subunit which halts the lengthening of the protein chain. My team determined that we would use a wide range concentration between 0 and 100 µg/ml of TNC. Given the working concentration from our lab instructor, the concentration of 15 µg/ml, we picked our volume of TNC below 30 µL and hypothesize that the TCN concentrations of more than 10µg/ml would be sufficient to deter the growth of EC. The results showed that with the increased
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Unfortunately, my team’s hypothesis that a concentration of 10µg/ml would be the least amount needed to stop EC was incorrect. According to the data, a concentration of 7.5 µg/ml TCN would be sufficient to halt the growth. That is a difference of 2.5 µg/ml which reduces the validity of this experiment.
Keywords: Tetracycline, Escherichia coli, gram-negative, gram-positive, concentration
Introduction
Everything we touch has the potential to kill us; yet, we don’t wear protection at a constant basis. Bacteria are found in the water we drink, the food we eat, and the air we breathe, but we still consume these organisms daily. Luckily, not all bacteria, also known as microbes, are harmful. However, some microbes, such as Escherichia Coli (EC), have the potential to damage or even kill humans and animals. EC is a gram-negative bacterium that resides in the gastrointestinal tract (Kaper, Nataro, & Mobley, 2004). Some strains aid and prevent the growth of other bacteria in the body. Unfortunately, other strains of the same bacteria have evolved to cause
Unfortunately, my team’s hypothesis that a concentration of 10µg/ml would be the least amount needed to stop EC was incorrect. According to the data, a concentration of 7.5 µg/ml TCN would be sufficient to halt the growth. That is a difference of 2.5 µg/ml which reduces the validity of this experiment.
Keywords: Tetracycline, Escherichia coli, gram-negative, gram-positive, concentration
Introduction
Everything we touch has the potential to kill us; yet, we don’t wear protection at a constant basis. Bacteria are found in the water we drink, the food we eat, and the air we breathe, but we still consume these organisms daily. Luckily, not all bacteria, also known as microbes, are harmful. However, some microbes, such as Escherichia Coli (EC), have the potential to damage or even kill humans and animals. EC is a gram-negative bacterium that resides in the gastrointestinal tract (Kaper, Nataro, & Mobley, 2004). Some strains aid and prevent the growth of other bacteria in the body. Unfortunately, other strains of the same bacteria have evolved to cause