The results support the first part of the hypothesis. The enzyme sample that was put into the hot water bath (80 ºC) had the smallest change in the rate meaning that there is no new products produced by the tyrosinase enzyme. No change in color presents that the substrates were not changed into the melanin product during the duration of 8 minute. The hot water bath was successful in denaturing the enzyme as represented by figure 1. The ice bucket samples (2 ºC) had the most change in the average absorbance over time presenting that tyrosinase enzyme was active in the reaction and could produce products from the substrates. The room temperature water bath (31 ºC) then had the next steepest slope out of all the other results as shown by figure
