If the pH solution in which the tyrosinase enzyme is different, then there will be different amounts of product concentration. This will be measured with the spectrophotometer. Different enzymes work faster with different pH buffers. According to research, “ the optimal pH for tyrosinase and catechol oxidation is 6-7” Therefore, using different buffers will result in different spectrophotometer readings. The optimal pH of 6-7 will cause the enzyme activity to go faster. Solutions with a pH of 4(acidic), 7(neutral), and 10(basic) will be tested with the tyrosinase and catechol. Each solution will be tested twice for validity. The solution with the same pH as water will produce a better environment for the enzyme to react. This reaction was previously proven in experiment
If the pH solution in which the tyrosinase enzyme is different, then there will be different amounts of product concentration. This will be measured with the spectrophotometer. Different enzymes work faster with different pH buffers. According to research, “ the optimal pH for tyrosinase and catechol oxidation is 6-7” Therefore, using different buffers will result in different spectrophotometer readings. The optimal pH of 6-7 will cause the enzyme activity to go faster. Solutions with a pH of 4(acidic), 7(neutral), and 10(basic) will be tested with the tyrosinase and catechol. Each solution will be tested twice for validity. The solution with the same pH as water will produce a better environment for the enzyme to react. This reaction was previously proven in experiment