When a blank containing boiled (denatured) enzyme instead of an active enzyme could be used but it would not be the better blank to use in this experiment. The better blank solution would have been the one with active enzyme because it would cause the spectrophotometer to measure the absorbance of every component in the blank except the substrate, creating a 100% transmittance. This allow the spectrophotometer to read everything that was not in the blank when the actual sample is inserted in because it would “blank” out all the components in the “blank” solution and only read the “uncommon” component. With the active enzyme, the spectrophotometer would “blank” out the enzyme and only read the substrate absorbance because according to the lab
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To increase the concentration of the substrate, leave the concentration of enzyme constant and add more volume of substrate in different tubes and then test of the absorbance values. Then using the standard curve, convert the absorbance values to concentrations and then plot them to look for a trend of increase. For example, set different increasing amount of substrates like Tube 1 would have 0.5mL, Tube 2 would 0.6mL … etc and see which volume of substrate would cause an increase in the production of …show more content…
A major area where enzymes are present a lot in is the food industry. A lot of the food we eat contain enzymes. Lactase is an enzyme that a lot of people know about. It cuts lactose into its smaller sugars: galactose and glucose. When the small intestine of a human does not produce a sufficient amount of this enzyme, this condition is known as the lactose intolerant, which is when a human has discomfort in the form of cramps, gas and diarrhea when they eat/drink milk products. Lactase is also used to make lactose-free products for people with this condition. Many other enzymes are present in the foods we eat and they all have different functions. These enzymes include hemicellulose, lipase, and amylase which are used in the baking industry along with proteases and carbohydrases which are used in the meat processing industry. Enzymes are also used in the medicine industry. Enzymes can be used to treat disorders such as “low blood pressure, myocardial infarction, tachycardia, head or spinal injuries, there are chances of formation of blood clots due to stagnation of blood inside the blood vessels,” enzymes are used to break down the clots so blood can flow better. Digestive enzymes like papain are given for patients to take orally after eating to make for easier digestion. In humans, there is an enzyme called Acrosome present in sperm to help them penetrate the uterine tissue so
To increase the concentration of the substrate, leave the concentration of enzyme constant and add more volume of substrate in different tubes and then test of the absorbance values. Then using the standard curve, convert the absorbance values to concentrations and then plot them to look for a trend of increase. For example, set different increasing amount of substrates like Tube 1 would have 0.5mL, Tube 2 would 0.6mL … etc and see which volume of substrate would cause an increase in the production of …show more content…
A major area where enzymes are present a lot in is the food industry. A lot of the food we eat contain enzymes. Lactase is an enzyme that a lot of people know about. It cuts lactose into its smaller sugars: galactose and glucose. When the small intestine of a human does not produce a sufficient amount of this enzyme, this condition is known as the lactose intolerant, which is when a human has discomfort in the form of cramps, gas and diarrhea when they eat/drink milk products. Lactase is also used to make lactose-free products for people with this condition. Many other enzymes are present in the foods we eat and they all have different functions. These enzymes include hemicellulose, lipase, and amylase which are used in the baking industry along with proteases and carbohydrases which are used in the meat processing industry. Enzymes are also used in the medicine industry. Enzymes can be used to treat disorders such as “low blood pressure, myocardial infarction, tachycardia, head or spinal injuries, there are chances of formation of blood clots due to stagnation of blood inside the blood vessels,” enzymes are used to break down the clots so blood can flow better. Digestive enzymes like papain are given for patients to take orally after eating to make for easier digestion. In humans, there is an enzyme called Acrosome present in sperm to help them penetrate the uterine tissue so