Types Of Organisms Within An Unknown Broth Culture Essay

1078 Words Nov 18th, 2015 null Page
This lab experiment was conducted in order to identify and confirm the presence of three types of organism within an unknown broth culture. The organism that was being tested fall under the categories of gram-positive, gram-negative paracolons, and gram-negative coliform. Gram positive organism is known to have a thick cell wall made up of peptidoglycan, which are cross-link sugar chains with peptide bonds (Carson 13). Gram-positive bacteria are found within the phylum Firmicuites (Slonczewski & Foster 94). While gram-negative, have thin layers of one or two peptidoglycan cell wall, this type of bacteria are found within the phylum Proteobacteria (Slonczewaki & Foster). The gram positive and negative characteristic derived from a staining technique that was developed by Hans Christian Gram (Carson 13). This technique help applies a different stain to an organism in order to help classify or determine the organism of interest base on cell wall composition. Diverse type of antibiotics interact differently with different groups of organism, this is pertinent information when it comes to prescribing antibiotics. Organism of a particular gram stain are known to share certain characteristic. This is of great importance in clinical settings, because this technique can help reduce ambiguity during the diagnosis process. In essence, to know the type of gram bacteria you are looking for within a sample can help narrow the choices down to a particular type of organism. Just like the…

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