The Test Of Biochemical Tests Essay example

1092 Words Oct 16th, 2015 5 Pages
Biochemical tests are used to detect the presence of different kinds of organic molecules, carbohydrates, lipids, nucleic acids, and proteins. These tests include the Benedict’s Test, Iodine Test, Sudan III Stain Test, Biuret Test and many others. With these tests, the nutrients from an unknown solutions can be identified in the solution.
The Benedict’s test is used for identifying reducing sugars, which are simple sugars that include monosaccharides and some disaccharides. The Benedict’s reagent is made of Copper (II) sulfate ion which reduces to Copper (I) during the test. It is made by adding copper sulfate with sodium citrate and carbonate that is dissolved in 400mLs of water (Benedict’s Test). The chemical reaction within this test is oxidation, which involves the loss of electrons. The solution and Benedict’s reagent is boiled in a water bath and slowly undergoes color changes from green, orange, red and finally brown (Biologically Important). These color changes wholly depend on the amount of sugar present in the solution because the aldehyde groups in the reducing sugars causes these changes. Based on the color, the quantity of the amount of reducing sugars can be identified. Green means there is 0.1 to 0.5 percent sugar in solution, yellow is 0.5 to 1 percent sugar is present, orange is 1 to 1.5 percent sugar is present, red is 1.-1.5 percent sugar is present, and lastly, brick red means that more than 2 percent sugar is present (Benedict’s Test for Reducing…

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