Name: Drasti Patel
ID #: 20575017
Partner: Sijia Luo
Course: BIOL 130L – 019
Tuesday, 7:30 Room # 151
TA: Ashutosh Patel, Doshina Naila
Date of Experiment: September 16, 2014
Date of Submission: September 23, 2014
Introduction:
The objective of this lab experiment is to identify different macromolecules by carrying out three different tests such as iodine test, Benedict’s test and the biuret test. These three tests are going to examine different properties of macromolecules such as their bonds, and functional groups to identify the solution as one of the four macromolecules.
The iodine test is used to determine the presence of polysaccharides such as starch and glycogen in the solution (Harisha, 2006). If the solution turns blue/black in colour …show more content…
These solutions include glucose, maltose, honey, lactose and beer. There was precipitation formed in these solutions, which resulted in the colour change indicating that sugar must be present in these solutions for this to occur (Nigam et.al, 2003). Monosaccharides such as glucose, maltose and lactose are all defined as simple sugars and since they showed a positive result in this test, it indicates that the Benedict’s test was performed correctly (Alberts et. al, 2014).
The objective of the biuret test was to find solutions that contain protein. The solutions that turn violet in colour display the presence of protein in them (Rocco, 2006). As shown in Table 3 above, we can see that two of the solutions we used in this lab experiment turned violet including protein solution and the unknown solution. This indicates the presence of protein in both of these solutions. We know that protein solution was the positive control of this test and since it had a colour change, it indicates that the biuret test was done