Essay The Concentration Of Hemoglobin And Iron Concentration

1772 Words Nov 14th, 2016 8 Pages
Abstract: Hemoglobin is an iron-containing protein in red blood cells that enable the red blood cell to bind oxygen in the lung and transport the oxygen to tissues and organs throughout the body. Oxygen binds reversibly to these iron atoms and is carried throughout the blood. As part of the experiment, we computed the concentration of hemoglobin and iron in a crystalline blood sample by measuring the absorbance of the sample as methemoglobin and cynamethemoglobin at 450-650nm. Furthermore, the hemoglobin and iron concentration were also measured by means of volumetric analysis and the Wong method. The concentration of Hemoglobin and iron obtained in the experiment was in good agreement with their corresponding literature value. The computation for the error analysis suggests that the error in the concentration with respect to the absorbance contributed highly to the result, because without it, the concentration of hemoglobin and iron in the blood sample will either be highly overestimated, underestimated or even incorrect. This work validates the literature values for the amount of hemoglobin and irom in a crystalline blood sample and it shows that both the Spectrophotometric analysis, the Wong method, and the volumetric analysis are effective method that can be used to predict the concentration of hemoglobin in a sample.

Introduction
Hemoglobin has a polypeptide chains that consist of four proteins, two alpha chains and two beta chains, each has ring-like heme group that…

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