b. Add two drops of 6.0M HCl(aq), 0.5mL 10% Hydroxylamine-HCl, six drops 2.0M Sodium Acetate, and 1.0mL 0.1% 2,2-Dipyridyl solution to the test tube. c. Fill the test tube to the 10mL mark with DI water and the color will fully develop in 15 minutes d. Prepare the four other standard solutions with appropriate amounts of the solutions. 9. The sample uses 2.0mL from the volumetric flask with the dissolved Fe and add the appropriate reagents. 10.…
Procedure 1- Set up the DAQ to output port 2 to send a binary data. 2- Run the LabView in continues mode, change the numerical input on the control panel, and observe the LEDs on the terminal block between (PB0- PB7).…
Purpose and Background Cells divide in two ways, mitosis and meiosis. Mitosis is used to produce cells that are genetically identical to the parent cell for growth, asexual reproduction, or repair after injury. Cells that are produced by mitosis are diploid, meaning that they have two complete sets of chromosomes, one from each parent. Meiosis is used to produce haploid cells that have only one set of chromosomes, a mix of chromosomes from both parents. Meiosis produces cells that are genetically unique from their parent cells.…
The optimal wavelength of maximum cranberry juice absorption was determined to be 525 nm. The calibration curve could then be created by measuring the absorbance of known concentrations, 5% to 25%, of cranberry juice at 525 nm, and generating a calibration curve of absorbance vs concentration. The absorbance of the cranberry apple juice was then measured at the wavelength of maximum absorbance. Absorbance is a measure of the amount of light that passes through a sample at a selected wavelength. The absorbance is measured by the spectrophotometer 20 which functions by emitting a visible light source through the sample.…
Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…
Abstract This experiment was a study of the different properties of a lipid bilayer. We used fluorescent microscopy to study the lateral diffusion of lipids and binding properties biotin and anti-biotin. The effect cholesterol has on the fluidity of the membrane was tested using Fluorescent Recovery After Photobleaching (FRAP). We used a Nikon Eclipse 50i upright fluorescent microscope with a Digital Sight DM-2MBW CCD camera to take images of the bilayer after photobleaching.…
Biology 15 Lab # 4 Professor Passerini September 23, 2015 Scot Albert Lab #4 Questions 1a,b,c, 2, 3a,b,c, 4, 5a,b,c, 7, 11c, d, e, 12a,b ---------------------------------------------------------- 1- a-They are found primarily in the thylakoid membranes. b-No. Cyanobacteria do not have distinct nuclei.…
The purpose of this experiment was to discover if Crayola Washable Markers are really nontoxic, or even as safe as the dyes used in food. To answer this question, we used paper chromatography to identify the dyes in blue, orange, and green Skittles Sweets and Sours candy and Crayola Washable Markers. In this paper chromatography experiment, the mixture to be separated was spotted 1.5 centimeters from the bottom of chromatography paper, which was then dipped in the solvent, a 0.1 percent salt solution. The solvent then moved up the filter paper via capillary action and carried the mixture's pigments with it (Rosen and Gothard, 2010). Each pigment has a unique retention factor or Rf, a ratio between how far a pigment travels to the distance…
Results In the lab, 478 trials were conducted using a total of 956 crickets. The 478 pairs resulted in 264 wins for the resident cricket and 214 wins for the intruding crickets. These results produced a chi squared value of 5.230. The p-value calculated from this lab was .022.…
Visual comparison of the available solid and aqueous compounds to the unknown was done first so as to eliminate unnecessary testing. A 30.0 g/mL solution of the unknown was made in a beaker by dissolving 1.000 g of the unknown in 30.0 mL of distilled water. Before weighing out 1.000 g of the unknown, the scale was zeroed out with a piece of weighing paper (square cut-out of normal paper) on it. Using a scoopula, 1.000 g was the compound was carefully placed on the weighing paper and measured to exactly 1.000 g. These same measurements and procedures were used when solutions of calcium chloride and nitric acid were created using separate beakers. Portions of each solution were then poured into test tubes (exact measurements were not necessary for this step).…
The Identification of Two Unknown Bacteria Mixtures Through the Method of Characteristic Testing and Elimination Khoa Vuong TA: Charlie Roll Micro 3301 4/11/24. Abstract: There is a surplus amount of microorganisms that are out there in the world. A single swab of any surface, including the human body, will yield many different types of microorganisms. Once grown on a nutrient-rich medium, the identification of these microorganisms can be made by isolating pure colonies and performing and using various plates and broth and recording its characteristics.…
Introduction For this lab, we were given two unknowns that we had to identify. One of the unknowns was live, meaning that we had to use morphological and biochemical methods to understand and discover what the organism was; and the other unknown was a digital unknown, that was identified through the genetic method, where we had access to a file with a DNA sequence and had to decode through a website that was given to us by the professor. We were given three milestones that we had to complete, two were naming our unknowns, and one was naming the arrangement of the unknowns. To complete these steps, we needed to work independently, using the skills that we learned in class to figure out our unknowns. Identification of Unknown Using Morphological…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
From here, the scientist utilized the colorimeter to find the absorbance of this solution in collusion with the absorbance plot for Copper (II) Nitrate solutions of various molarities. From the linear fit of the Beer’s Law plot, calculations for the determination of the molarity and percent mass of copper were executed. Because the absorbance and concentration variables have a direct relationship in the Beer’s Law Equation, the linear fit was an accurate method for calculating the unknown. From the graph, it was observed that as the concentration (or molarity) of the Copper (II) Nitrate solution increased, the absorbance of the solution increased. The scientist was able to relate this finding back to her conclusions from the previous activity to realize that the dark the shade of blue, the less color variance that was reflected and the higher the absorbance.…
Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size. Horizontal gel slabs are commonly used on conducting gel electrophoresis.…