Pseudomonas Research Paper

1104 Words 5 Pages
Introduction:
The name Pseudomonas is derived from the greek and latin words which means “false unit“ (pseudo = false, greek; monas = single unit, latin). “Monas” was used in the early history of microbiology to define single-celled organisms. In 1786 Otto Friedrich Müller, from Copenhagen, classified the bacteria and named the pseudomonads, they came into the group of vibriones (which was defined as group of shaking bacteria). Many years later it was detected that Pseudomonas are motile. They therefore were given the name “Pseudo”, because they appeared to be shaking but in reality they were motile. Because of their widespread occurrence in water, the pseudomonads were categorized and named as one of the first microorganism groups. Pseudomonas
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Pseudomonas aeruginosa can be cultured on;
• MacConkey agar
• Blood agar
• Luria Broth (LB) and Luria Agar (LA) Media
• Cetrimide Selective Agar
• Tryptic Soy Agar
• Cystine Lactose Electrolyte-Deficient (CLED) Agar
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Since P.aeruginosa is frequently found in the hospital environment and in the normal stool flora, it is difficult to trace the source of infection and/or colonization unless a complete study is undertaken.(Teplitz et al., 1964). The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and yielded more readable pyocin inhibition zones. Seventy-eight of 1,084 isolates (7.2%) were untypable. In the second part of the study, P. aeruginosa was isolated from 110 patients (5.4% of all admissions) in a 3-month period and typed with the revised method. Twenty pyocin types were identified, 10 of which were obtained from five or more patients. P. aeruginosa was isolated from 45 of 353 environmental samples, including water fountains, ice machines, bar soaps, and germicide solutions for toilet brushes. Twenty percent of the environmental samples were untypable but, among typable strains, the five most common environmental strains were the same as the strains most frequently isolated from patients. The organism was frequently isolated from noses (39%), throats (39%), and stools (29%) of patients with P. aeruginosa infections or colonizations in urine,

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