Occurrence Of Microrna Expressions Using Two Macronutrient Deficiencies
882 Words Mar 6th, 2015 4 Pages
Occurrence of microRNA expressions using two macronutrient deficiencies presented to Arabidopsis Thaliana
Arabidopsis Thaliana was the model chosen for this experiment. It was used for miRNA expression because its entire genome is already sequenced (Weems 362-369). It is also easy to grow in difficult conditions and has a short lifespan. This all makes it an easy plant to work with for research (Weems 362-369). The macronutrients phosphorus and sulfur were used for the study because they are essential for plant growth (Axtell). Both phosphorus and sulfur are components of coenzymes and proteins (Axtell). Controlling gene expression via miRNA is the plant’s way of responding to limiting levels of these essential macronutrients. miRNA works by binding to single stranded mRNA which creates double stranded RNA. Thus the plant identifies the new double stranded mRNA as inferior and attacks it.
For the experiment, there were four microRNAs used including miR156, miR395, miR398, and miR399. miR156 was the control and has no nutrient requirement so it would be expected to not show any changes in nutrient levels (Hsieh 2120-2132). miR395 depends on sulfur concentrations and targets mRNAs involved in sulfur metabolisms (Hsieh 2120-2132). miR398 is regulated when there is Copper/Zinc starvation, targeting the enzyme copper/zinc superoxide dismutase (Bouché 684– 686). miR399 is up-regulated during phosphate starvation and thus targets phosphorus uptake and…