Investigating The Separation Of A Mixed Substance Essay

891 Words Sep 20th, 2016 4 Pages
What is Chromatography?

Chromatography is a laboratory term for the separation of a mixed substance. These mixed substances are separated based on their physical or chemical properties; some of these properties include charge, molar mass and properties in liquid. During a chromatography separation, a series of processes including a stationary (permeable solid that is packed into a metal tube, glass tube or the walls of an open-tube capillary) and mobile phase (a liquid solvent or mixture of solvents that flows through the stationary phase caring the stationary phase along with it), these phases can be either liquid or gas. In the chromatography, the eluting solvent is used in addition with the stationary phase to help the flow of molecules through the stationary phase. “Molecules that are strongly attracted to the stationary phase will be retarded or retained, relative to molecules that are not strongly attracted to the stationary phase.” Choosing the right mobile and stationary phase is needed to ensure ta proper separation.

Different types of Chromatography:

There are many different types of chromatography that follow the same type of principle of separation but differ in attractions, chemical composition and geometry in terms of mobile and stationary phases. Some popular chromatography include paper chromatography, thin layer chromatography (TLC), column chromatography, gel-filtration chromatography, affinity chromatography and antigen-antibody or…

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