Investigating The Correlation Between The Wavelength Of Light Absorbed And The Color Of A Compound

1288 Words Nov 16th, 2016 6 Pages
Discussion/Scientific Explanation: In order to successfully analyze colas, one must become familiar with a Spectrophotometer 20. In order to do this, one must figure out determine the relationship between the wavelength of light absorbed and the color of a compound. Next, one must determine a way to relate the amount of light absorbed with the concentration of the solution. After gaining knowledge about these two parts of the lab, one will be able to determine how a Spectrophotometer 20 can be helpful in determining the concentration of phosphate in colas.3 A spectrophotometer is used to obtain measurements of the amount of light that passes through a object and to measure the amount of light absorbed by a certain material. In order to do this, it is important for one to learn about a spectrophotometer. To use a spec 20, one must plug it in and allow it to heat up for a few minutes. Next, one must enter the desired wavelength and insert a blank cuvette filled with distilled water into the cell compartment.3
For Week 1, five cuvettes were given and were filled with the following: water, red food coloring, green food coloring, a mixture of red and green food coloring, and potassium permanganate. Each material was diluted with water and placed in a separate cuvette so that the spectrophotometer could accurately reveal the absorbance. The cuvette that was filled with water was used as a blank to zero out the spectrophotometer. Each cuvette was then inserted into the…

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