Ethical statement
This work, performed at early stages of chick development, does not need ethical approval from review board institution or ethical commission.
Eggs and embryos
Fertilized chicken eggs, Gallus gallus, were incubated between 4.5-5.5 days in a 49% humidified atmosphere, at 37°C. Embryonic lungs were dissected under a stereomicroscope (Olympus SZX16, Japan) and staged in b1, b2 and b3 according to the number of secondary buds formed: 1, 2 or 3, respectively [Moura et al. 2011]. Dissected lungs were processed for in situ hybridization or for in vitro lung explant culture.
This work, performed at early stages of chick development, does not need ethical approval from review board institution or ethical commission.
Eggs and embryos
Fertilized chicken eggs, Gallus gallus, were incubated between 4.5-5.5 days in a 49% humidified atmosphere, at 37°C. Embryonic lungs were dissected under a stereomicroscope (Olympus SZX16, Japan) and staged in b1, b2 and b3 according to the number of secondary buds formed: 1, 2 or 3, respectively [Moura et al. 2011]. Dissected lungs were processed for in situ hybridization or for in vitro lung explant culture.