Essay On Catechol Experiment

The null hypothesis for this experiment reads a change in the substrate, Catechol, concentration then there will be a change in the rate of reaction. The alternate hypothesis states if there is a change in Catechol concentration then there will be no change in the rate of reaction. To explain the null, if one chooses to change the amount of substrate in each trial then you should expect to see a change in a number of products produced in those trails. I predict that the trials with less concentration of the substrate will yield a lower rate of reaction and vice versa. In the experiment, test tube 2 was filled with 6mL of the substrate, Catechol, and test tube 3 was filled with 4mL of the substrate. During the first 30 seconds, the mean Benzoquinone concentration of test tube 2 and test tube 3 were respectively, .074mM and .077mM. At the 90 seconds mark the mean Benzoquinone concentration for test tube 2 was .178mM and .13mM for test tube 3. Lastly, at 180 seconds the mean Benzoquinone concentration for test tube 2 was .188mM and .136mM for test tube 3. This data proves the major trend that shows as you increase substrate concentration you will also see an increase in your rate of reaction over time by increasing your Benzoquinone …show more content…
The difference in the slope of the 3 line proves that the ending concentrations could not be the same therefore the rate of reactions have to be affected, again rejecting the alternate hypothesis. This can also go to prove my prediction that the trials with less concentration of the substrate will yield a lower rate of reaction and vice versa. To simply put it when there is less substrate in the test tube there is also fewer chances of the added enzyme reacting with the substrate causing the rate of reaction to be