Effect Of Lb/X-Gal On Bacterial Growth

Decent Essays
The plate labeled LB/AMP/X-gal-plasmid and the plate labeled LB-plasmid display that AMP (ampicillin) and X-gal prevent cells without plasmid from growing. The necessary factors for bacterial growth are growing room, appropriate temperature, and minerals. Likewise, any substance that hinders growth will be malignant to the plasmid. In this experiment, that substance was ampicillin.
The plate LB/Amp/X-gal+plasmid and other plate LB/Amp/X-gal–plasmid did not have any growth. None of the plates had growth except for LB+plasmid and LB/AMP/x-gal+plasmid.
The plasmid was not ampicillin resistant in the experiment my class conducted because it did not have the conditions to adapt that way. In order for resistance against ampicillin, it would be essential

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