Cold Calcium chloride (CaCl2) was added to two transformation tubes. These tubes were labeled “+p” and “-p” respectively. E. coli was streaked on a starter plate and incubated at 37°C overnight. Then, the E. coli was swabbed from the plate and added into each tube. The pGLO plasmid was only added to the +p tube. After the preparation of the tubes, both tubes were incubated on ice for 15 minutes. Then, they were heat shocked at 42°C for 50 seconds and placed back on the ice for 2 minutes. Lastly, nutrient broth was added to each tube and both tubes were incubated at room temperature for 10 minutes. The E. coli in the tube containing the pGLO plasmid (+pGLO) was swabbed onto 2 separate plates with a sterile loop. One plate contained Laurie
