Ampicillin Effect Lab Report

Decent Essays
The Ampicillin Effect of Escherichia coli in a
Beta Lactamase plate and non Beta Lactamase plate.

Jimena Cárcamo

Lab 4
Concepts in Biology 173 C
October 6, 2014

INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing microorganism, the culture has to be completely pure. The best technique to preserve the culture pure is by avoiding bacteria in the environment to contaminate the culture, and to prevent the culture in contaminating the environment.
There are many
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The antibiotic in the Beta Lactamase provides the only results in the experiment. The top Ampicillin disk has a diameter of 2.7 centimeters. The middle Ampicillin disk has a diameter of 2.7 centimeters. The bottom Ampicillin disk has a diameter of 2.9 centimeters. The non Beta Lactamase had no results due to the fact that, it had bacteria all over the plate. The Ampicillin did not have any effect in the non Beta Lactamase plate, for it was covered with colonies. The Ampicillin did have a huge effect in the Beta Lactamase, for it destroyed the E.coli surrounding each disk. Thus, our data indicated that the Ampicillin has more effect in the Beta Lactamase, and it is not useful in the non Beta …show more content…
The results can be evaluated that ß Lactam is an antibiotic that is resistant to penicillin and the Ampicillin is an antibiotic in the penicillin group. The Ampicillin had no effect in the non Beta Lactamase, because it had no ß Lactam that made it easier for the E.coli to spread around the plate. The Ampicillin in the Beta Lactamase has ß Lactam that is resistant to penicillin, which makes the Ampicillin react to the E.coli and prevent it from spreading. With the results, we can conclude that our hypothesis is true, because the beta plate did reduce antibiotics effectiveness, but only in the Beta Lactamase

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