The Ampicillin Effect of Escherichia coli in a
Beta Lactamase plate and non Beta Lactamase plate.
Jimena Cárcamo
Lab 4
Concepts in Biology 173 C
October 6, 2014
INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing microorganism, the culture has to be completely pure. The best technique to preserve the culture pure is by avoiding bacteria in the environment to contaminate the culture, and to prevent the culture in contaminating the environment.
There are many different ways to prevent microbial growth. The most common form to destroy microbial growth is by using antibiotics. Antibiotics are substances produced by living organism, to kill or inhibit the growth of other organisms (Lab Sheet, 5). Antibiotics are normally altered to fit specific characteristics that require a natural form. Antibiotics can also be changed to reduce toxicity in an organism. Antibiotics are essential to combat bacteria. In this experiment, the bacterium that is being texted is Escherichia coli also known as E.coli. E.coli is a bacterium that normally lives in the intestines. E.coli it is not harmless, but when infected it can cause diarrhea or other intestinal problems (Mayo Clinic, 2014). The antibiotic that is used is Ampicillin. Ampicillin is an antibiotic in the penicillin group, and it fights bacteria in the body (Drugs, 2014). Beta Lactamase is an enzyme resistant to ß Lactam antibiotics, which is a ring penicillin and cephalosporin (Saunders, 2007). The hypothesis for this experiment is “That the beta plates would have reduced antibiotic effectiveness”. METHODS For our experiment, we decided to use two plates: one with beta lactamase and the other plate did not contain any beta lactamase. In both of the petri dishes we dip a swab into the broth culture of E.coli. We used the swab to smear the E.coli all over the …show more content…
The antibiotic in the Beta Lactamase provides the only results in the experiment. The top Ampicillin disk has a diameter of 2.7 centimeters. The middle Ampicillin disk has a diameter of 2.7 centimeters. The bottom Ampicillin disk has a diameter of 2.9 centimeters. The non Beta Lactamase had no results due to the fact that, it had bacteria all over the plate. The Ampicillin did not have any effect in the non Beta Lactamase plate, for it was covered with colonies. The Ampicillin did have a huge effect in the Beta Lactamase, for it destroyed the E.coli surrounding each disk. Thus, our data indicated that the Ampicillin has more effect in the Beta Lactamase, and it is not useful in the non Beta …show more content…
The results can be evaluated that ß Lactam is an antibiotic that is resistant to penicillin and the Ampicillin is an antibiotic in the penicillin group. The Ampicillin had no effect in the non Beta Lactamase, because it had no ß Lactam that made it easier for the E.coli to spread around the plate. The Ampicillin in the Beta Lactamase has ß Lactam that is resistant to penicillin, which makes the Ampicillin react to the E.coli and prevent it from spreading. With the results, we can conclude that our hypothesis is true, because the beta plate did reduce antibiotics effectiveness, but only in the Beta Lactamase
Beta Lactamase plate and non Beta Lactamase plate.
Jimena Cárcamo
Lab 4
Concepts in Biology 173 C
October 6, 2014
INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing microorganism, the culture has to be completely pure. The best technique to preserve the culture pure is by avoiding bacteria in the environment to contaminate the culture, and to prevent the culture in contaminating the environment.
There are many different ways to prevent microbial growth. The most common form to destroy microbial growth is by using antibiotics. Antibiotics are substances produced by living organism, to kill or inhibit the growth of other organisms (Lab Sheet, 5). Antibiotics are normally altered to fit specific characteristics that require a natural form. Antibiotics can also be changed to reduce toxicity in an organism. Antibiotics are essential to combat bacteria. In this experiment, the bacterium that is being texted is Escherichia coli also known as E.coli. E.coli is a bacterium that normally lives in the intestines. E.coli it is not harmless, but when infected it can cause diarrhea or other intestinal problems (Mayo Clinic, 2014). The antibiotic that is used is Ampicillin. Ampicillin is an antibiotic in the penicillin group, and it fights bacteria in the body (Drugs, 2014). Beta Lactamase is an enzyme resistant to ß Lactam antibiotics, which is a ring penicillin and cephalosporin (Saunders, 2007). The hypothesis for this experiment is “That the beta plates would have reduced antibiotic effectiveness”. METHODS For our experiment, we decided to use two plates: one with beta lactamase and the other plate did not contain any beta lactamase. In both of the petri dishes we dip a swab into the broth culture of E.coli. We used the swab to smear the E.coli all over the …show more content…
The antibiotic in the Beta Lactamase provides the only results in the experiment. The top Ampicillin disk has a diameter of 2.7 centimeters. The middle Ampicillin disk has a diameter of 2.7 centimeters. The bottom Ampicillin disk has a diameter of 2.9 centimeters. The non Beta Lactamase had no results due to the fact that, it had bacteria all over the plate. The Ampicillin did not have any effect in the non Beta Lactamase plate, for it was covered with colonies. The Ampicillin did have a huge effect in the Beta Lactamase, for it destroyed the E.coli surrounding each disk. Thus, our data indicated that the Ampicillin has more effect in the Beta Lactamase, and it is not useful in the non Beta …show more content…
The results can be evaluated that ß Lactam is an antibiotic that is resistant to penicillin and the Ampicillin is an antibiotic in the penicillin group. The Ampicillin had no effect in the non Beta Lactamase, because it had no ß Lactam that made it easier for the E.coli to spread around the plate. The Ampicillin in the Beta Lactamase has ß Lactam that is resistant to penicillin, which makes the Ampicillin react to the E.coli and prevent it from spreading. With the results, we can conclude that our hypothesis is true, because the beta plate did reduce antibiotics effectiveness, but only in the Beta Lactamase