The experiment aims to test the hypothesis that was raised in the last 60 years of the transformation principle by Avery, MacLeod and McCarty whom used the pneumococcal types. However, in this experiment the E.coli cells were used to determine the transformation principle. The strains were mixed properly with various enzymes under certain conditions of which cells can adapt, the participation of enzymes- Lysozymes, DNAse, protease and RNAse, and a buffer indicate the polymer in the cell that is responsible for transformation or on which transformation partakes. Results had shown that the DNA is the transformation polymer, which recombination has occurred between the E.coli ampicillin sensitive strain and the ampicillin resistant strain. On
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This was avoided by introducing the ampicillin strain into the E.coli cells which made them ampicillin resistant. Thus by transfection of the DNA, E. coli was able to take up the strain into its genome and recombination occurred as the cell became ampicillin resistant. That is why colonies were able to form on the agar plates.
All the experimental plates had colonies including the agar plate of the enzyme of DNAse. Which simply contradicts the transformation principle, this effect could arose by systematic errors that have occurred during the experiment, that is, the enzyme DNAse was inactive therefore could not interfere with the growth of the bacterial cells and/ or could be result of not preparing the experiment in sterile conditions. However, goal standard plates were set up for comparison purposes- the DNAse had no colonies of bacterial cells indicating preferable results for hypothesis of the transformation principle. According to goal standards, the DNAse destroyed the DNA of the ampicillin resistant strain of which the recombination of dna could not occur and the e. coli was still ampicillin sensitive thus when exposed to the agar plate it led to direct apoptosis as the genome had no gene for ampicillin. Therefore, the goal standards approves the evidence of the last 60
This was avoided by introducing the ampicillin strain into the E.coli cells which made them ampicillin resistant. Thus by transfection of the DNA, E. coli was able to take up the strain into its genome and recombination occurred as the cell became ampicillin resistant. That is why colonies were able to form on the agar plates.
All the experimental plates had colonies including the agar plate of the enzyme of DNAse. Which simply contradicts the transformation principle, this effect could arose by systematic errors that have occurred during the experiment, that is, the enzyme DNAse was inactive therefore could not interfere with the growth of the bacterial cells and/ or could be result of not preparing the experiment in sterile conditions. However, goal standard plates were set up for comparison purposes- the DNAse had no colonies of bacterial cells indicating preferable results for hypothesis of the transformation principle. According to goal standards, the DNAse destroyed the DNA of the ampicillin resistant strain of which the recombination of dna could not occur and the e. coli was still ampicillin sensitive thus when exposed to the agar plate it led to direct apoptosis as the genome had no gene for ampicillin. Therefore, the goal standards approves the evidence of the last 60