Csrn1 Case Study
However, the usage of some synonymous codons generated by these base substitutions significantly differed compared to the consensus ones of Variant B: TCT1260 (relative synonymous codon usage value [RSCU], 0.81) vs. TCC1260 (1.50) vs. TCG1260 (0.66) for Ser; CCG1311 (0.50) vs.CCA1311 (1.12) for Pro (fig. 2 and 3C; Tang et al. 2007). Of the three downstream bases that were clonally mutated in the lineage A-V sequences, the first two bases comprised Leu (T1346TA) and Thr (AC1353C) codons of gag, as well as Phe (TT1346T) and His (CAC1353) codons of rt, in Variant B (fig. 1). The Leu TTA codon had the lowest frequency among the six synonymous codons (RSCU, 0.55), whereas ACC was the most frequent one for Thr (1.36). The TG1346T (1.14) in the prominent active lineage of Variant A (lineage A-V) was the more frequent codon than its synonymous counterpart to encode Cys. Therefore, considering the positions of the CHCC Gag-motif codons and the hungry Leu codon (fig. 3C), the short nucleotides CCC*TTTA encompassing the characteristic C/T1344-insertion site (*) might act as the signal for ribosomal -1 frameshifting in the precedential CsRn1 copies (Jacks et al. 1988; Hatfield and Oroszlan 1990; Kalmykova et al.