Collection And Identification Of An Environmental Bacteria Essay

1818 Words Oct 28th, 2015 8 Pages
Collection and Identification of an Environmental Bacteria to the Genus level

Introduction: Bacteria can be found virtually anywhere, and interaction with these organisms occurs daily (Fitzpatrick & Allison, 2014). However, though the interaction between human and bacterium is continual, it is not always known what type of bacteria a person is coming into contact with. Therefore it is important to understand, and to have knowledge about, the characteristics of bacteria in our environment. As part of a microbiology lab course, students were assigned to collect and identify an environmental sample down to the genus level. For this particular experiment a skin bacterial sample, taken from the arm, was selected and put through different differential tests to discover the genus of the particular bacterium. Not only was this particular sample chosen as part of the curriculum of a microbiology course, but it was also chosen to further understand the organism in which the human body shares a symbiotic relationship with (Fitzpatrick & Allison, 2014).
Materials:
• Environmental Unknown sample
• TSA plates
• Gram Stains: Crystal Violet, Grams Iodine, 95% ethanol, and Grams Safranin
• Microscope Slides
• Fluid Thioglycollate broth tubes
• Catalase test: microscope slides and Hydrogen peroxide
In order to identify the unknown bacterium collected, only three tests were needed to be performed. The first test included Gram staining. Gram Staining is a differential stain that determines…

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