Anti C Alloantibodies : The Most Probable Antibody Present Essay

1057 Words Nov 14th, 2016 5 Pages
Anti-C alloantibodies are the most probable antibody present in the patient’s sample. Reagent screening cells 2 and 3 were not reactive at immediate spin, 37°C, or in the AHG phase. The screen ruled out antibodies to D, c, E, e, k, Kpb, Jsb, Fyb, Jka, Jkb, Lea, Leb, P1, M, N, S, s, Lub, and Xg*a antigens. In all positive screening cells, reactions occurred at immediate spin, 37°C, and AHG phases; however, screening cells with positive reactions did not react with same strength.
The panel ruled out antigens V*, Cw, K, Fya, and Lua. In panel cells, reactions occurred at immediate spin, 37°C, and AHG phases. This indicates the presence of IgG class antibodies, as IgM class antibodies only react at immediate spin. The C antigen was expressed in panel cells 1, 2, 5, 9, and 10 and weakly expressed in panel 11. Panel cells 1,2, 5, 9, and 10 reacted with patient’ serum. Panel cells 3, 4, 6, 7, 8, 11, 12, 13, 14, 15, and 16 did not react with the patient’s serum. With the exception of panel cell 11, patient serum reacted with the panel cells expressing the C antigen and did not react with panel cells that did not express the C antigen. Panel 11’s weak expression of the C antigen could not cause visible hemagglutination, even after the addition of AHG. The P value was calculated to be 2.8*10-6. This value does support the probability that the antibody identification is correct. A p value that is less than or equal to 0.05 is the minimum value at which an antibody identification is…

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