The anti-benzoylecgonine (cocaine) monoclonal antibody [IP3G2] (mouse IgG1, catalog no. GTX44358, lot 821400072, 3.75 mg/ml) was purchased from Gene Tex (Irvine, CA, USA). The second anti-benzoylecgonine monoclonal antibody [IP3G2] (mouse IgG1,catalog no. MAB4029, lot 8H23323, 3.75 mg/ml) was purchased from Acris Antibodies (Herford, Germany).
The secondary antibody, a polyclonal anti-mouse IgG from sheep (catalog no. R1256P, lot 21481, 2.2 mg/ml), was obtained from Acris Antibodies (Herford, Germany).
Cocaine hydrochloride, cryst, extra pure, L55041262 was obtained from Merck, KL3aA, 64271, Darmastadt, Germany. Benzoylecgonine (tetrahydrate), batch 02-D-23 was obtained from National Measurement Institute, North Ryde, NSW, 1670, Australia. …show more content…
N,N –dicyclohexylcarbodiimide (DCC, puriss), tetrabutylammonium borohydride (purum, ≥97%) , hydrogen peroxide solution ≥30% (TraceSELECT®) were from Fluka (Sigma-Aldrich, Taufkirchen Germany). The following chemicals were also purchased from Sigma-Aldrich: N-hydroxysuccinimide (NHS, 98%) N, N-dimethylformamide (DMF, ≥99.5%), sodium phosphate dibasic dehydrate (purum p.a.), sodium phosphate monobasic dihydrate (BioUltra, ≥99%), sodium chloride (BioUltra, ≥99.5%), potassium phosphate monobasic (BioUltra, ≥99.5%), potassium phosphate dibasic (BioUltra, 99%), potassium sorbate (purum p.a.), for the preparation of buffers. Additional, TweenTM 20 (pure) and 3,3_,5,5_-tetramethylbenzidine (TMB, research grade) were also obtained from Serva. GuardianTM Peroxidase Conjugate Stabilizer/Diluent was obtained from Toronto Research Chemicals (North York, Canada). Sulfuric acid 95–97% (both Baker-analyzed grade), isopropanol (Baker HPLC analyzed) and methanol (HPLC gradient grade) were purchased from Mallinckrodt Baker (Griesheim, Germany). Ethyl acetate and NaHCO3 were supplied by Merck KGaA (Darmstadt,
The secondary antibody, a polyclonal anti-mouse IgG from sheep (catalog no. R1256P, lot 21481, 2.2 mg/ml), was obtained from Acris Antibodies (Herford, Germany).
Cocaine hydrochloride, cryst, extra pure, L55041262 was obtained from Merck, KL3aA, 64271, Darmastadt, Germany. Benzoylecgonine (tetrahydrate), batch 02-D-23 was obtained from National Measurement Institute, North Ryde, NSW, 1670, Australia. …show more content…
N,N –dicyclohexylcarbodiimide (DCC, puriss), tetrabutylammonium borohydride (purum, ≥97%) , hydrogen peroxide solution ≥30% (TraceSELECT®) were from Fluka (Sigma-Aldrich, Taufkirchen Germany). The following chemicals were also purchased from Sigma-Aldrich: N-hydroxysuccinimide (NHS, 98%) N, N-dimethylformamide (DMF, ≥99.5%), sodium phosphate dibasic dehydrate (purum p.a.), sodium phosphate monobasic dihydrate (BioUltra, ≥99%), sodium chloride (BioUltra, ≥99.5%), potassium phosphate monobasic (BioUltra, ≥99.5%), potassium phosphate dibasic (BioUltra, 99%), potassium sorbate (purum p.a.), for the preparation of buffers. Additional, TweenTM 20 (pure) and 3,3_,5,5_-tetramethylbenzidine (TMB, research grade) were also obtained from Serva. GuardianTM Peroxidase Conjugate Stabilizer/Diluent was obtained from Toronto Research Chemicals (North York, Canada). Sulfuric acid 95–97% (both Baker-analyzed grade), isopropanol (Baker HPLC analyzed) and methanol (HPLC gradient grade) were purchased from Mallinckrodt Baker (Griesheim, Germany). Ethyl acetate and NaHCO3 were supplied by Merck KGaA (Darmstadt,