Material and Method
Subjects and samples-
This was a case-control study conducted in King George’s Medical University, a tertiary-care teaching institute, and Ram Manohar Lohia Hospital, a tertiary-care government non-teaching institute, in Lucknow, Uttar Pradesh, India. A total of 1118 subjects were enrolled including 559 cases and 559 controls. Cases were defined as mothers (age 18-40 years) of live preterm neonates (birth at less than 37 weeks of gestation). Controls were eligible mothers who delivered a neonate at or after 37 weeks of gestation, consecutive to an enrolled case. Mothers who gave birth to twins or those with neonates having congenital anomalies were excluded from the study.
Total 3ml venous blood was taken from each subject for further molecular study. Ethical clearance for the study was obtained from the Institutional ethical committee of King George’s Medical University. Written informed consent was obtained from participating mothers.
Data sources/ measurements-Demographic, environmental, clinical and physical examination findings were recorded for all the cases and controls. Data was …show more content…
Odds ratios (ORs) for this model with the corresponding 95 % confidence intervals (95 % CIs) were computed. Allele frequencies were assessed by counting alleles and calculating sample proportions. The allelic frequency distributions in the control and case groups were compared by the Pearson chi-squared test. In each group the allele distribution was checked for deviations from Hardy-Weinberg equilibrium using an exact test. The haplotypic association between the genes was constructed with the help of SNP version 2.0 software .Statistical analyses were performed with SPSS software, version 12. Results were considered statistically significant when the probability of findings occurring by chance was less than 5% (p<
Subjects and samples-
This was a case-control study conducted in King George’s Medical University, a tertiary-care teaching institute, and Ram Manohar Lohia Hospital, a tertiary-care government non-teaching institute, in Lucknow, Uttar Pradesh, India. A total of 1118 subjects were enrolled including 559 cases and 559 controls. Cases were defined as mothers (age 18-40 years) of live preterm neonates (birth at less than 37 weeks of gestation). Controls were eligible mothers who delivered a neonate at or after 37 weeks of gestation, consecutive to an enrolled case. Mothers who gave birth to twins or those with neonates having congenital anomalies were excluded from the study.
Total 3ml venous blood was taken from each subject for further molecular study. Ethical clearance for the study was obtained from the Institutional ethical committee of King George’s Medical University. Written informed consent was obtained from participating mothers.
Data sources/ measurements-Demographic, environmental, clinical and physical examination findings were recorded for all the cases and controls. Data was …show more content…
Odds ratios (ORs) for this model with the corresponding 95 % confidence intervals (95 % CIs) were computed. Allele frequencies were assessed by counting alleles and calculating sample proportions. The allelic frequency distributions in the control and case groups were compared by the Pearson chi-squared test. In each group the allele distribution was checked for deviations from Hardy-Weinberg equilibrium using an exact test. The haplotypic association between the genes was constructed with the help of SNP version 2.0 software .Statistical analyses were performed with SPSS software, version 12. Results were considered statistically significant when the probability of findings occurring by chance was less than 5% (p<