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As indicated in Table 1, the maximum wavelength was at 625 nm at 1.063 absorbance. The absorbance values from cuvettes #1 through #7 were recorded at 625 nm to create the concentration curve Figure 2. The concentration curve is conducted at the maximum wavelength to produce the optimal results for the diluted concentrations of Fast Green.The purpose of the graph is to locate the unknown; Beer-Lamberts law, the concentration is proportional to the absorbance. The slope of the trend line was y = 71.333x + 0.0014 and the length travelled through the sample was 1 cm, the absorbance at the unknown substance was 0.112. After calculating using Beer-Lamberts law, the unknown concentration was 0.001550474 mg/ml. Calculations are described in the Results section. As shown on the graph, a straight line is drawn from the absorbance value, 0.112, until it intersects with the trend line of the graph. The perpendicular line that corresponds with the abscissa is the concentration 0.001550474