An Isolated Strain Of Pseudomonas Sp Essay
To isolate the microorganism, they first collected a soil sample from the waste disposal site and inoculated the sample in a nutrient medium with a dye. Then they incubated and plated in agar with dye. The colonies that had a halo of decolorization were selected to be submitted to biochemical tests and 16S rDNA analyses that identified Pseudomonas sp. SUK1. The phylogenetic analyses showed a close relation with other strains that already have reports of a role in biodegradation of dye.
The study of acclimatation with increasing concentration of dyes showed that the microorganism were able to degrade the dye to 80% less after 114 h, even in concentrations of 5 g/L (Figure 1). The enzyme analyses showed the activity of lignin peroxidase, azoreductase and DCIP reductase. The toxicity study showed that development of two species of plants (S. vulgare and P. mungo) were more negatively affected by the dye Reactive Red 2 wastewater than with the contact with the wastewater after the proposed treatment. This result shows that this mechanism oof degradation generates products less toxic than the original compound.
The study of Min et al (2015) “A dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and b-ether…