DISCUSSION:
Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates.
To examine the antimicrobial activity of those isolates, in vitro inhibition by cross streak assay was performed, in cross streak method all the 5 isolates were able to inhibit the growth of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Salmonella enterica (NCTC 6017), and Bacillus cereus. Previous studies also revealed …show more content…
We found that isolates which produce higher amount of pyocyanin pigment were capable to highly inhibit the pathogenic microbe’s growth. In the quantification assay it showed that PS1 isolate produce maximum amount of pyocyanin and it was also highly capable of inhibit most of the pathogen and proved that pyocyanin’s antibacterial activity is concentration dependent (El-Fouly et al. 2015 ). Confirmation of pyocyanin was done using several task, where it was confirmed that the crude extract of the isolates surely have pyocyanin pigment. TLC, UV-Vis spectra suggests the characteristics of the pyocyanin compound, which in like manner matched with previous data’s. Pyocyanin is a hetero cyclic nitrogenous compound, the FT-IR spectra indicate the presence of –OH group (3400-3300 cm-1), C=N bonds (1590-1600 cm-1) and C-N (1280- 1250 cm-1) bonds, the presence of –CH3 group is confirmed with the –C-H stretches of the alkyl (methyl) group in the 1380-1400 cm-1 range (Stuart n.d.) As it has been clearly suggesting from this experiment, that this pyocyanin compound present on the crude extract, secreted by the P. aeruginosa can exhibit good antimicrobial …show more content…
et al., 2004. Glutathione and glutathione peroxidase in sputum samples of adult patients with cystic fibrosis. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 3, pp.119–124.
El-Fouly, M.Z. et al., 2015. Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa. Journal of Radiation Research and Applied Sciences, 8(1), pp.36–48. Available at: http://linkinghub.elsevier.com/retrieve/pii/S1687850714001095.
Essar, D.W. et al., 1990. Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: Interchangeability of the two anthranilate synthase and evolutionary implications. Journal of Bacteriology, 172, pp.884–900.
Fothergill, J.L. et al., 2007. Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC microbiology, 7, p.45.
Fuse, K. et al., 2013. Reduction of virulence factor pyocyanin production in multidrug-resistant Pseudomonas aeruginosa. Journal of Infection and Chemotherapy, 19, pp.82–88.
Hassan, H.M. & Fridovich, I., 1980. Mechanism of the antibiotic action of pyocyanine. Journal of Bacteriology, 141(1),
Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates.
To examine the antimicrobial activity of those isolates, in vitro inhibition by cross streak assay was performed, in cross streak method all the 5 isolates were able to inhibit the growth of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Salmonella enterica (NCTC 6017), and Bacillus cereus. Previous studies also revealed …show more content…
We found that isolates which produce higher amount of pyocyanin pigment were capable to highly inhibit the pathogenic microbe’s growth. In the quantification assay it showed that PS1 isolate produce maximum amount of pyocyanin and it was also highly capable of inhibit most of the pathogen and proved that pyocyanin’s antibacterial activity is concentration dependent (El-Fouly et al. 2015 ). Confirmation of pyocyanin was done using several task, where it was confirmed that the crude extract of the isolates surely have pyocyanin pigment. TLC, UV-Vis spectra suggests the characteristics of the pyocyanin compound, which in like manner matched with previous data’s. Pyocyanin is a hetero cyclic nitrogenous compound, the FT-IR spectra indicate the presence of –OH group (3400-3300 cm-1), C=N bonds (1590-1600 cm-1) and C-N (1280- 1250 cm-1) bonds, the presence of –CH3 group is confirmed with the –C-H stretches of the alkyl (methyl) group in the 1380-1400 cm-1 range (Stuart n.d.) As it has been clearly suggesting from this experiment, that this pyocyanin compound present on the crude extract, secreted by the P. aeruginosa can exhibit good antimicrobial …show more content…
et al., 2004. Glutathione and glutathione peroxidase in sputum samples of adult patients with cystic fibrosis. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 3, pp.119–124.
El-Fouly, M.Z. et al., 2015. Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa. Journal of Radiation Research and Applied Sciences, 8(1), pp.36–48. Available at: http://linkinghub.elsevier.com/retrieve/pii/S1687850714001095.
Essar, D.W. et al., 1990. Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: Interchangeability of the two anthranilate synthase and evolutionary implications. Journal of Bacteriology, 172, pp.884–900.
Fothergill, J.L. et al., 2007. Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC microbiology, 7, p.45.
Fuse, K. et al., 2013. Reduction of virulence factor pyocyanin production in multidrug-resistant Pseudomonas aeruginosa. Journal of Infection and Chemotherapy, 19, pp.82–88.
Hassan, H.M. & Fridovich, I., 1980. Mechanism of the antibiotic action of pyocyanine. Journal of Bacteriology, 141(1),