Micro Iii

Front 1

Genetic engineering of plants has so far produced

Back 1

pest resistant plants

plants that are herbicide resistant

plants with increased nutritional value

(all of the above)

Front 2

Restriction enzymes have proved so useful in manipulating DNA because

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They cut defined sites

The sticky ends make it very easy to allow recombination of any type of DNA

(A and B)

Front 3

Flourescence In Situ Hybridization (FISH)

Back 3

-Uses Labeled probe

-is useful in microbial ecology

-allows identification of particular bacterial groups in mixed samples

(B,C,D)

Front 4

When a vector that employs the LacZ' gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

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white colonies

Front 5

In order to get around the lack of ability of prokaryotes to remove introns from precursor RNA, it may be necessary to

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turn mRNA into cDNA

Front 6

Artificial chromosomes

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-are used to contain large inserts

-are available for use in bacterial, yeast, and mammalian cells

-(B, D)

Front 7

Introducing DNA into eukaryotic cells may use

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-the natural ability of viruses to infect a cell

-the natural ability of agrobacterium to transfer DNA into a cell

-electroporation

(A,B,C)

Front 8

Southern Blotting

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-provides information of presence or absence and size of the DNA of interest

Front 9

A dye often used for its case and sensitivity to visualize nucleic acid--?-- in gel electropherensis is

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ethidium bromide

Front 10

Dideosynucleotides

Back 10

-are useful in nucleic acid sequencing

-act as chain terminators

Front 11

Starting with a single piece of dsDNA, after 3 PCR cycles there are

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8 additional pieces of dsDNA

Front 12

Nucleotide array technology

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-may use a "DNA chip"

-attaches nucleotides to a solid support such as a silicon chip

-uses nucleic acid hybridization

Front 13

Mitochondrial DNA

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-is inherited from the mother

-shows the same sequence in mother, child, and natural grandmother

Front 14

Genetically Modified food has raised some concern because

Back 14

-it may contain some unanticipated allergens

-it may have some unintended environmental effects

-the modified DNA may transfer to other organisms

Front 15

From most general to most specific which is the correct order?

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kingdom, phylum, class, order, family, genus, species

(king phillip came over for ginger snaps)

Front 16

the name lactococcus (streptococcus) lactis

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indicates that spreptococcus is the old genus name

Front 17

intracellular gram-negative diplococci found in a urethral sample from a male is indication of

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Neisseria gonorrhea

Front 18

Mycobacterium tuberculosis is one of the few species of bacteria that

Back 18

are acid fast

Front 19

media that changes color as a result of the biochemical activity of growing bacteria

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usually contain a pH indicator in the media

Front 20

serological methods

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-are useful in identifying unknown bacterium

-rely on specificity of an antibody-antigen interaction

-may be simple and rapid

Front 21

pulse field electrophoresis and ribotyping

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both use restrictive enzymes

Front 22

phage typing

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is used to distinguish bacterial strains

Front 23

the ticks of a genetic clock are measured by the

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number of random mutations

Front 24

similarity coeffecients are used in

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numerical taxonomy

Front 25

to study the phylogeny of eukaryotes

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18s rRNA is used

Front 26

Disagreements between conclusion obtained from rDNA data and other techniqes may be explained by

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horizontal DNA transfer

Front 27

Fatty acid methylated esters

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are the form required for analysis by gas chromatography

Front 28

the methanogens

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-are part of the archaea

-oxidize hydrogen gas to produce methane

Front 29

hydrogen sulfide

Back 29

-is produced when sulfur compounds are used as terminal electron acceptors

-may react with iron to produce a black precipitate

-is produced by Desulfovibrio

-has a rotten egg smell

Front 30

The lactobacilli in their role as normal flora of the vagina, help the vagina resist infection by contributing to

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acidity of the vagina

Front 31

Which of the following contain bacteriochlorophyll?

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-chromatium, thiospirillum, thidictyon

Front 32

phycobiliproteins are

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-found in cyanobacteria

-used to gather wavelengths of light that are not well absorbed by chlorophyll

Front 33

the conversion of ammonia to nitrate could be accomplished by the presence of

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both nitrosomonas and nitrobacter

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pseudomonas

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-are resistant to many disinfectants and antimicrobials

-are mostly harmless except for opportunistic P.aeruginosa

Front 35

Members of the family enterobacteriaceae

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-include E. coli, enterobacter, salmonella, shigella

-include many medically relevant bacteria

-primarily reside in intestine tract of humans and animals

-are facultative anaerobes

Front 36

bacteria that may form endospores include

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clostridium and bacillus

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streptomyces

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-resemble fungi in their pattern of growth

-produce a nubmer of antibiotics

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rhizobium

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fix nitrogen inside nodules on the roots of legumes

Front 39

sphaerotilus and leptothrix are examples of

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sheathed bacteria

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bdellovibrio

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play on other bacteria

Front 41

reticulate and elementary bodies are two forms of

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chlamydia

Front 42

members of the archaes typically thrive in conditions of excess

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-heat

-acidity

-alkalinity

-salinity

Front 43

teh salem withc hunts may have been caused by

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-the ingestion of a poision produced by fungus

Front 44

Algae

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often grow in areas where other forms of life may not

Front 45

diatons are algea whose silicon dioxide containing shells are useful economically for?

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filters

Front 46

which dinoflagellate kills fish by dispersing toxins directly into the water?

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pfiesteria

Front 47

the ability to exist as either a trophozoite or a cyst is characteristic of many

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protozoa

Front 48

all fungi have _____ in their cell walls

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chitin

Front 49

fungi are classified according to their

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method of sexual reproduction

Front 50

fungi capable of dimorphism grow either as

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yeast like or mycelium

Front 51

lichens may be an association of

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algae and fungus

Front 52

mycorrhizas

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-are vital for the survival of lichens

-increase the absorptive ability of roots

Front 53

when cellular slime molds run out of food, they form a

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slug

Front 54

nematodes

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-are roundworms

-are found in the gi tract or the blood

Front 55

tapeworms

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-do not have a digestive system

-may be transmitted by eating undercooked meat