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Stats Flowchart

Hypothesis


Chose the needed comparisons


Decide if parametric tests are needed


Transform the data to fit parametric tests

Repeated Measures

Each subject is measured more than once


They can serve as their own control

Simplest Within Design

One group of subjects each individual measured twice at different times


Assess with a paired t-test

Sources of variability in Repeated Measures

Variability between treatments


Variability between delta scores (residual variability)

Variation in traditional ANOVA

SStotal composed of SSbetween (treatment) and SS within

Variation in Repeated Measures

SS total is comprised of SS treatment and SS residual


NO BETWEEN SUBJECTS VARIATION


F = MStreat/MSresidual


MS is assumption of population variance

Why Repeated Measures is Better

Make MS residual smaller by reducing variation between subjects

Reminder About Error Bars

Don't tell us much in a repeated measures design


Delta could be increased consistently each time

Degrees of Freedom in 1 way ANOVA

a= # of groups


Classic: (a-1), a(n-1)


Repeated Measures:


(a-1), (a-1)(n-1) - UNIVARIATE

Univariate and Multivariate Agree

They are both significant


Report univariate


Report either


Greenhouse-Geisser (more conservative)


Huynh-Feldt (more liberal)


Which you report depends on design

Uni and Multi Disagree

One is significant and the other is not


You may have violated assumptions


Best to report the multivariate if they slightly disagree


Or consult statistician if they really dont agree

Why repeated measures is alpha

You can greatly reduce residual error

2 Way Between DFs

Factor A: (a-1)


Factor B: (b-1)


AxB: (a-1)(b-1)


Residual: ab(n-1)

2 Way Within DFs

A: (a-1) resid: (a-1)(n-1)


B : (b-1) resid: (b-1)(n-1)


AxB: (a-1)(b-1)


resid: (a-1)(b-1)(n-1)

ANOVAs on Percentages

Each condition needs replicates


Parametric assumptions must be met


Beware of skewness near 0 and 100

When to NOT do ANOVA on percentage

When there is no within cell variability


Instead: Chi-Square or Fischer exact test

MCTs and Repeated ANOVA

Dunnetts - MSerror greatly reduced, becomes super powerful


Tukeys- Super powerful as well


(First two cannot be performed by most stats programs)


Bonferroni- with laired t tests easiest with stats as long as comparisons arent over 8

MCTs and Normalization

Raw Data: Repeated measures and any MCT


Final group mean and SEM multiplied by 100/MEAN:


Can do all tests, will provide same p as variation between and within are multiplied by a constant


Each raw value multiplied by 100/control value:


No variance in control group


Cannot perform ANOVA or MCTs


Can still do paired t with bonferroni


Simpsons Paradox

Direction of a correlation can be reversed by a lurking variable


Success rates for one treatment can be better in both cases but reversed when the cases are combined

Defining the Variability

Between Subjects - Different folks not used in repeated measures


Between Treatment - Variation because of the treatment


Residual - How an individual responds to a given tretment