Chemistry

Front 1

Kjeldahl's procedure for total protein is based upon the premise that

Back 1

the nitrogen content of proteins is constant

Front 2

the term "biuret reaction" refers to

Back 2

coordinate bond btwn Cu2+ & carboxyl and amino groups of biuret

Front 3

which statement about the biuret reaction for total protein is true

Back 3

polypetides & compounds w/ repeating imine groups react

Front 4

which of the following protein methods has the highest analytic sensitivity

Back 4

Folin lowry

Front 5

which of the following statements regarding proteins is true

Back 5

total protein & albumin are about 10% higher in ambulatory patients

Front 6

hyperalbuminemia is caused by

Back 6

dehydration syndromes

Front 7

high serum total protein but low albumin is usually see in

Back 7

multiple myeloma

Front 8

which of the following condtx is most commonly ass w/ elevated level of total protein

Back 8

malignancy

Front 9

which of the following dyes is the most specific for mz of albumin

Back 9

BCP - bromcresel purple

Front 10

which of the following factors is most likely to cause a falsely low result when using the BCG dye binding for albumin

Back 10

the presence of penicillin

Front 11

at pH 8.6 proteins are _____ charged & migrate towards the ______

Back 11

negatively
anode

Front 12

electrophoretic movement of protein toward the anode will be made to decrease by increasing the

Back 12

ionic strength of the buffer

Front 13

at pH 8.6, the cathodal movement of gamma (Y) globulin is caused by

Back 13

electroendomosis

Front 14

which of the following condtx below will prevent any migration of protein across an electrophoretic support medium such as agarose

Back 14

loss of contact btwn a buffer chamber & the medium

Front 15

which of the proteins listed below has the highest pH

Back 15

IgG

Front 16

which of the proteins listed below migrates in the beta region at pH 8.6

Back 16

transferrin

Front 17

which of the following is one advantage of high resolution (HR) agarose electrophoresis over lower current electrophoresis

Back 17

high resolution procedures detect monoclonal & oligoclonal bands at a lower concentration

Front 18

which of the following condtx is ass w/ Beta gamma (BY) bridging

Back 18

hepatic cirrhosis

Front 19

which support medium can be used to determine the molecular weight of a protein

Back 19

polyacrylamide gel

Front 20

which of the following stains is used for lipoprotein electrophoresis

Back 20

oil red O

Front 21

which of the following serum protein electrohporesis result suggested an acute inflammatory process

Back 21

albumin - decrease
alpha 1 - increase
alpha 2 - increase
Beta - normal
gamma - normal

Front 22

which of the following condtx is usually ass w/ an acute inflammatory pattern

Back 22

myocardial infarction (MI)

Front 23

what is the clinical utility of testing for serum prealbumin

Back 23

serial low level indicate compromised nutritional status

Front 24

which serum protein should be measured in patient suspected of having Wilson's disease

Back 24

ceruloplasmin

Front 25

a pat w/ hemolytic uremic syndrome ass w/ septicemia has a normal haptoglobin level although the plasma free HGB is elevated & hemoglobinuria is present - which test would be more appropriate than haptoglobin to mz this pat's hemolytic episode

Back 25

hemopexin

Front 26

quantitative determination of HGB A2 is best performed by

Back 26

column chromatography

Front 27

HGB F concentration is usually mz by

Back 27

alkali denaturation of HGB A and HGB A2

Front 28

Select the correct order of HGB migration on agarose or cellulose acetate at pH 8.6

Back 28

-- C-->S-->F-->A +

Front 29

which of the following abnormal HGBs migrates to the same position as HGB S on agarose or cellulose acetate at pH 8.6

Back 29

HGB D punjab

Front 30

which HGB is a Beta-Delta chain hybrid & migrates to the same position as HGB S at pH 8.6

Back 30

HGB lepore

Front 31

select the correct order of HGB migration on CITRATE agar at pH 6.2

Back 31

-- F-->A--->S--->C +

Front 32

which HGB separates from HGB S on citrate (acid) agar but not on agarose or cellulose agar

Back 32

HGB D punjab

Front 33

in double immunodiffusion reactions the precipitin band is

Back 33

concave to the protein of greatest molecular weight

Front 34

which statement best describes immunofixation electrophoresis (IEF)

Back 34

proteins are separated by electrophoresis followed by overlay of monospecific anti-immunoglobulins

Front 35

which of the following statement regarding the identification of monoclonal proteins by IFE is true

Back 35

when testing for monoclonal gammopathy, both serum & urine must be examined

Front 36

which statement regarding IFE is true

Back 36

CSF should be concentrated 50 to 100 fold before performing IFE

Front 37

which of the following statement regarding IFE is true

Back 37

oligoclonal banding is seen in the CSF of more than 90% of multiple sclerosis cases

Front 38

detection of isoemz bands following electrophoresis is facilitated by

Back 38

adding substrates to th gel & mz catalytic activity

Front 39

which isoemz of CK has the fastest electrophoretic mobility at pH 8.6

Back 39

BB

Front 40

capillary electrophoresis differs from agarose gel elecrophoresis in which respect

Back 40

a stationary support is not used

Front 41

select the order of mobility of lipoprotein electrophoresed on cellulose actate or agarose at pH 8.6

Back 41

-- Chylomicrons-->Beta---> pre-Beta--> alpha +

Front 42

following ultracentrifugation of plasma, which fraction correlates w/ pre-B lipoprotein

Back 42

VLDL - very low density lipoprotein

Front 43

select the lipoprotein fraction that carriers most of the endogenous triglycerides

Back 43

VLDL

Front 44

the protein composition of HDL is what percentage by weight

Back 44

50%

Front 45

broad (floating) B lipoprotein occurs in familial B-dyslipoproteinemia (type III hyperlipoproteinemia) & consist of

Back 45

IDL

Front 46

which of the following mechz accounts for the elevated plasma levels of B-lipoproteins seen in familiar hypercholesterolemia (type II hyperlipoproteinemia)

Back 46

ApoB 100 receptor defect

Front 47

which emz defcy is most commonly ass w/ familial hypertriglyceridemia, which is ass w/ fasting plasma chylomicrons (type I hyperlipoproteinemia)

Back 47

postherparain activated lipoprotein lipase defncy

Front 48

which of the following condtx is most consistently ass w/ secondary hypercholesterolemia (type II lipoproteinemia)

Back 48

hypothyroidism

Front 49

which of the following is ass w/ Tangier's disease

Back 49

apoprotein A-I defncy

Front 50

what is the formula for LDL calc

Back 50

LDL chol = TC - (HDL + TG/5)

Front 51

which of the following formulas correctly estimates the BLDL cholesterol

Back 51

serum trig divided by 5
TRIG/5

Front 52

which is the screening procedure for adults recommended by National Cholesterol Ed Program, to eva risk for atherosclerosis

Back 52

lipid profile, fasting every 5 years

Front 53

what is the most appropriate fasting procedure when a lipid study of trig, TC, HDL & LDL tests are ordered

Back 53

12 hours, nothing but water allowed

Front 54

select the cutpoint value for serum or plasma LDL chol in a person w/ history of coronary heart dz in whom therapeutic lifestyle changes should be initiated

Back 54

>100 mg/dL

Front 55

what is the HDL chol cutpoint recommend by the NCEP

Back 55

<40 mg/dL

Front 56

an EDTA blood sample is collected from a nonfasting person for a CBC, the doc collected the sample from the femoral vein cz venipuncture from the arm was unsuccessful. he called the lab 15 mins after the sample arrive & request a lipid studying inc TRIG, TC, HDL & LDL - which test results should be used to eva the pat's risk for coronary artery disease

Back 56

TC and HDL cholesterol

Front 57

which of the following dz is caused by a deficiency of SPHINGOMYELINASE

Back 57

Niemann Picks disease

Front 58

which method is considered the candidate reference method for TRIG measurement

Back 58

CDC modification of Van Handel & Zilversmit

Front 59

which of the following emz is common to all emz methods for TRIG measurement

Back 59

glycerol kinase

Front 60

select the reagent needed in the coupling emz rtx used to generate a color product in the cholesterol oxidase method for chol

Back 60

Phenol

Front 61

which of the following method for HDL chol is the reference method

Back 61

ultracentrifugation

Front 62

cholesterol esterase is used in emz assays to

Back 62

hydrolyze fatty acids bound to the 3rd carbon atom of cholesterol

Front 63

which of the following reagents is used in the direct HDL chol method

Back 63

sulfate cyclodextrin

Front 64

what do "direct" or homogenous method for LDL chol ass have in common

Back 64

all use a detergent to facilitate selective reactivity w/ reagent emz

Front 65

what is the purpose of the saponification step used in the Abell Kendall method for chol mz

Back 65

convert chol esters to free cholesterol

Front 66

lipoproteins (a) or Lp(a) is important when elevated in serum because

Back 66

is an independent risk factor for atherosclerosis

Front 67

which type of dietary fatty acid is not ass w/ increase in serum LDL chol production

Back 67

monosaturated cis-fatty acids

Front 68

lab results shows as follow what do you do next
TC = 220 mg/dL
HDL chol = 40 mg/dL
TRIG = 420 mg/dL

Back 68

perform a direct LDL chol assay

Front 69

a person has a fasting TRIG of level of 240 mg/dL, the doc wishes to know the patients non HDL chol level, what chol fraction should be measured

Back 69

TC and HDL cholesterol