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28 Cards in this Set

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1 ml added to 51 ml gives a dilution of --
1/52; 1:51
how to prepare microscope for storage
- loop cord around eyepiece
- specimen holder mechanism centered on stage
- put to lowest objective
- lower stage as far down as possible
- put dust cover on
variable you have most control on
independent variable
variable you are trying to measure
dependent variable
pipette suitable for measuring volumes
serological pipette
pipette suitable for transferring volumes
pasture pipette
3 main factors of activity of an enzyme
- enzyme concentration
- pH
- temperature
the nature of an enzyme activity is --
specific
catalyst that increases the rate of reaction by reducing activation energy
enzyme
At high activation energy --
- more products
- higher absorbance
- lower transmitance
enzyme activity will reach to its maximum at --
optimal condition
a blank solution contains --
all compounds except for what we are trying to measure
function of an enzyme is directly related to--
its shape
the solution you put the solute in
solvent
dissolves in solvent
solute
3 characteristics of a given protein that can differ from those of another protein
- solubility
- size
- charge
ion exchange chromatography separates molecules by --
charge
purpose of "salting out"
concentrates protein and could be utilized as a purification step
why do we need to desalt protein before we start the batch ion exchange chromatography step
to concentrate the protein and so the salt doesn't interfere with the charge of the protein
dilution factor equation
final volume of dilution/original sample volume
the diameter of the circle of view you can see when looking down a microscope
field of view
the higher the magnification --
the smaller the field of view
as you go from low to high power, the light intensity --
decreases
isotonic solution
the net movement of water molecules is zero
hypotonic solution
more water will move inside the cell than moving outside, therefore cell wall will swell up and burst
hypertonic solution
the concentration of solute molecules outside the cell are more than inside, therefore water will move out from the cell and cell will shrink
osmosis
movement of water molecules across a semi permeable membrane from lower concentration of solutes to high concentration
diffusion
movement of solute molecules across a semi permeable membrane from higher concentration of solutes to lower concentration