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55 Cards in this Set

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Explain speciation

- geographical isolation (physical barrier)


- different selection pressures e.g. climate, disease, predation, competition


- mutations > natural selection


- advantageous allele = ^ chances of survival & reproduction


- lack of allele = death > less competition for resources


- allele frequency increases > becomes more common


- species becomes GENETICALLY DISTINCT


- X reproduce to create fertile offspring > no longer the same species (reproductive isolation)

How do seed banks & zoos contribute to scientific research

(Reintroduction into the wild - both)


Zoos:


- studying animal behaviour


(increases our knowledge of animals’ physiological & nutritional needs)


Seedbanks:


- medicine, disease resistance


- new crops used for new materials

What is the role of water in plants

- photosynthesis > creating it own food


- maintaining structural rigidity (> water exerts pressure in vacuhole)


- to regulate temperature (> evaporation from leaves helps cool plants down) > maintaining internal conditions


- to transport minerals in xylem

Similarities between the xylem & phloem

- contain dead & living cells


- form a part of the vascular bundle


- show primary & secondary growth


- complex tissues composed of more than one cell type


- involved in transporting substances

Differences between sclernchyma & phloem

- S is for support P is not


- S cells are thickened w/ lignin whilst in P this is not the case


- P is for transport of organic solutes S X involved in transport


- P contains dead & living cells whilst S only contains dead cells

Similarities between xylem & Sclerenchyma

- both provide support


- both contain cells thickened w/ lignin


- both are a part of the vascular bundle


- both contain dead cells

Differences between xylem & phloem

- X transport water & minerals whilst P transports organic solutes e.g. glucose


- X contains mainly dead cells whilst P does not


- X provides support whilst P does not

Similarities between sclerenchyma & phloem

- both are a part of the vascular bundle


- both contain dead cells

Differences between xylem & sclerenchyma

- X transports water & minerals whilst the P does not


- Only X has pits


- Only X can do autolysis (breaking down)

Importance of nitrate ions in plants

- to create DNA (proteins & enzymes)


- to create chlorophyll


- required for growth + fruit & seed production

Importance of calcium ions in plants

- for growth


- important component of the plant cell wall > calcium pectate forms a part of the middle lamella + maintains cell membrane permeability

Importance of magnesium ions in plants

production of chlorophyll (> photopigment needed for photosynthesis)

What conditions are required for bacterial growth

- energy source e.g. glucose > respire & grow


- moist > water available for bacterial cell functions


- oxygen > aerobic respiration > energy


- warm > temperature increases rate of reactions in bacteria


(temperature & pH > effects enzyme activity > effects metabolic processes)

How do seedbanks store seeds

Dry cold conditions

4 problems with re-introducing species into the wild after captive breeding (4 marks)

- cruel to keep animals in captivity


- may find it difficult to communicate & reproduce w/ members of the same species


- may introduce a new disease into the habitat

Xylem

- dead cells (no cytoplasm) joined end to end to create long, hollow, uninterrupted tubes (Have no end walls)


- thickened with lignin > strength (thick walls)


- support


- transports water & minerals


- pits > no lignin


- transports upwards only (from the roots)

Phloem

- dead & living cells


- translocation of organic solutes (e.g. sucrose)


- sieve tube elements


- companion cells


- sieve plates (has holes wall extending across the sieve tube elements) allow organic solutes through

Sieve tube elements

Structure: no nucleus, thin cytoplasm, few organelles


- living cells (> but unable to carry out ‘living processes’)


- joined end by end to form sieve tubes


- have holes in them allowing solutes through


- paired with a companion cell

Companion cells

- carries out living functions for BOTH itself & sieve tube element e.g. respiration


- provide energy for active transport

Sclerenchyma fibre

- dead cells


- long tube like structure with hollow lumen & end walls


- provides support


- thickened with lignin > strength

How can you measure species diversity in 2 different areas?

- species richness (count no. of dif species in the area)


- Index of diversity (calculate abundance/ count no. of individuals in species)

What is genetic diversity? How can genetic diversity be measured?

- variety of alleles within a species


- Hardy Weinberg principle, heterozygosity index

Explain the classification system

- grouping together based on similarities


- 8 levels: domain, kingdom, phylum, class, order, family, genus, species


- down hierarchy > fewer organisms in each group


- species > reproduce to make fertile offspring


- genotype & phenotype

Naming a species

‘Homo sapiens’


First word = genus name (homo)


Last word = species name (sapiens)


Binomial (two-word) system


Unique Latin name for each species

When is the Hardy Weinberg principle valid/true?

- large population


- no emigration, immigration, mutations, natural selection


- random mating is required

Why is the hardy Weinberg principle not true at these times?

- It predicts allele frequency will stay constant across generations (from one generation to the next)


- all these factors do not allow for this statement to be true

What is the difference between geographic and reproductive isolation?

- Geographic isolation is a type of reproductive isolation


- reproductive isolation is the major mechanism that leads to speciation

Why was the classification system changed

new evidence from molecular phylogeny > suggested organisms had more in common > archae, bacteria & eukaryota

Describe the arrangement of cellulose in a plant cell wall & explain how this relates to the properties of plant fibres (2)

- net like arrangement of cellulose microfibrils


- strength of microfibrils in this arrangement increases plant strength

microfibrils

Describe secondary thickening of plant cell walls and explain how this relates to the properties of plant fibres (2)

- secondary thickening = production of another cell wall between the plant cell wall and cell membrane


- secondary cell wall is thicker (& has more lignin than a normal cell wall) giving plant fibres lots of strength

Starch (structure)

- amylose (1-4, unbranched, coiled > compact)


- amylopectin (1-4,1-6, branched, long) > easily releases energy


- alpha glucose

Bond joining cellulose chains/molecules

Hydrogen bonds

Cellulose (structure)

- beta glucose (inverted from the previous molecule at each binding point > stops it cooling)


- long, unbranched chains


- 1-4 glycosidic bonds


- straight chains

Starch (function)

- amylose > storage > compact > more can fit into a smaller space


- amylopectin > branched (& 1-6 bonds)> glucose can be easily hydrolysed

Cellulose (function)

- unbranched, long straight chains > stronger intermolecular bonds > rigid


- microfibrils formed due to h bonds between chains > strength


- net like arrangement of microfibrils (cross over each other) > strength

How would you identify sclerenchyma fibres phloem sieve tubes and xylem vessels and their location within stems through a light microscope?

- use a mounted needle to remove vascular bundle from (rhubarb) tissue


- place on microscope slide


- cover with methylene blue (leave for 5 minutes)


- place dilute glycerol on fibres


- mount under coverslip


- look at under microscope (changing the magnification)

What bonds are there between monomers within cellulose

Glycosidic bonds as the monomers are beta glucose

Investigate plant mineral deficiencies

- IV: minerals present in solution


- DV: physical characteristics of the plant e.g. growth


- control variables: exposure to sunlight, type of plant, time growing, volume of solution


- fill test tube with solution > cover top with cling film > put seed on top


- repeats & mean

Investigate tensile strength of plant fibres

- IV: type of plant


- DV: amount of mass needed for fibre to break (strength)


- Control variables: length of fibre, size of (same) individual masses, thickness of plant fibre


- Connect between 2 clamp stands > record when breaks


- repeats & mean


Contemporary drug testing (pre-clinical)

animal & human cells/tissues

Why is it tested on healthy volunteers first?

- establish if there are dangerous side effects


- different doses to determine safe dose

Outline historic drug testing methods

- tested on ppl with the disease


- trail & error > determine correct dosage


- extract (not pure drug)

Contemporary drug testing (clinical phases)

1) small sample of healthy individuals


2) small sample of ppl with disease


3) large sample of ppl with disease (+one grp receives new treatment & other grp receives existing treatment > see if better)


(3 stage process)

How to increase validity of drug tests

- use placebo (see if the treatment has an effect > accounts for psychological effects i.e. improvement shown because the patient thinks they’re being treated)


- use a double blind trial (neither patient nor doctors know who has the drug/ placebo > reduces bias > attitudes of patient & doctors x effect results)

What is the difference between historic and contemporary drug testing? (Min 5)

1) pure drug vs extract > valid


2) larger samples > reliable


3) tested on animals and human cells before on humans (pre clinical trials) > safer


4) double blind trials/ placebo used > validity


5) control over factors in tested cohort e.g. age > valid


6) regulated by legislation > safer


7) results analysed statistically > reliable

Investigate the antimicrobial properties of plants

- IV: type of plant (garlic, mint, control)


- DV: antibiotic properties > size of zone of inhibition


- control variables: temperature of petri dish, volume of plant extract,


- disc of absorbent paper dipped into extract, petri dish, inverted & incubated at 25 degrees (for 1/2days),


- aseptic techniques used to prevent contamination (e.g. sterilising equipment)


- repeats & means

How to ensure contamination doesn’t occur on agar plates?

- sterilised equipment/ disinfect work spaces (destroys microbes)


- work under a Bunsen burner (> hot air rises so microbes in the air move away from the plate)

Why may plant extracts be soaked in ethanol (antimicrobial practical)

This will extract the antimicrobial substances as they are soluble (dissolve) in ethanol

Why are plant materials more sustainable?

- renewable > plants can be re-grown > future generations have access to same materials


- biodegradable > can be broken down by microbes > dnt remain in the earth

How can the use of plant fibres contribute to sustainability?

- less fossil fuels used to make materials


- renewable > can be regrown


- biodegradable > can be broken down by microbes


- easier to grow & process > cheaper

How can the use of starch contribute to sustainability?

- makes bioplastics & bioethanol


- less fossil fuels used to make materials


- renewable > can be regrown


- biodegradable > can be broken down by microbes

Advantages of seedbanks

- cheap (less space)


- little maintenance


- stored in dry & cool place (dnt need very specific conditions)


- less likely to be damaged by disease, natural disaster

Disadvantages of seedbanks

- test for viability > expensive & time consuming


- seeds in remote locations > hard to acquire + costly


- expensive to store many seeds

Why are organisms re-introduced into the wild?

- unethical for them to live in a cage


- no.s of that species are critically low in the wild > endangered > to conserve the species


- restoring habitats that have been lost > helps organisms relying on these plants

How do zoos educate people on conservation?

- empathy > allowed to touch the animals


- more likely to support conservation