Theory of Photometry Essay

1397 Words May 23rd, 2014 6 Pages
Biochem 300 Lab
Fall 2012 Lab #1
Theory of Photometry
In this lab we are conducting a series of test to verify the theory of photometry. A spectrophotometer is used to measure the absorbance of light of a sample. The absorption of light mechanism consist of a light bulb, a compartment to place the test tube, a wavelength selector, a slit a light detecting photocell and a meter to show the measurement of the sample. The compound can absorb a photon of light, if the energy of the photon, is equal to the amount of energy needed to emit electrons of the sample to a higher energy orbital. The mechanism works by turning a light bulb, which permits light to go through a prism that is set in a specific wavelength, then this light is
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This number was average of all my results with a error margin of ± 0.001.
Extinction Coefficient of PNP c = (0.140/6.0) x 1000 = 23.33 x 1mole/106 = 2.33 x 10-5 M
E = 0.500/(2.33 x 10-5 M)(1.4cm) = 15328.0 = 1.53 x 104 M-1 x cm-1
Worksheet # 2 λ 340
350
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Absorbance
0.150
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0.360
0.500
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1.000
1.100
1.000
0.900
0.750
0.560

λ
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Absorbance

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