This lab was conducted in order to determine the molarity of sucrose concentration where the mass of 2cm^3 of Classic Russet potato segments and pear segments does not change, representing an isotonic concentration. By calculating the change of mass of the potato segment and pear segments prior to being placed in the sucrose solution against the mass after being in the sucrose solution for 45 minutes. Our goal was to determine what type of osmotic concentration was created as compared to the potato and pear segments. The results of the lab indicated six different osmotic concentrations. According to the data, the isosmotic concentration, of a potato segment is around 0.22M. At this sucrose concentration the sucrose solution is isotonic …show more content…
Next, the mass was calculated for each cubes to the nearest 0.01g. The mass was recorded on a table that also showed the sucrose molarity. Next, the potato cubes were placed into different sucrose concentrations, 0.0M, 0.2M, 0.4M, 0.6M, 0.8M, and 1.0M. The potato cubes were then submerged in the various sucrose concentration solutions for 45 minutes. After the time is up, the potato cubes were removed from their concentration. The excess solution was blotted off with a dry paper towel. The potato cubes were then placed on a scale and each were measured for their mass. Finally, we calculated the percent change in mass was calculated by diving the change in mass by the initial mass then multiplying by one hundred. The same procedures were followed with the pear segments, with the exception that instead of a glucose molarity, sucrose would be used to six different levels being 0.0M, 0.20M, 0.40M,0.80M, and …show more content…
The percent change in mass of the potato segments is positive when placed in concentrations of 0.0 and 0.1M of glucose. At these two concentrations, a hypertonic environment was observed within the segment cells. The percent change in mass of the potato cells is negative when placed in concentrations of over 0.3M to 0.4M, 0.5M, and 0.6M of sucrose. At these three concentrations, a hypotonic environment was observed within the cell. According to the data, a line of best fit crosses the x-axis where an isotonic relative osmotic concentration is achieved. An isotonic solution was achieved when the glucose solution is around