Research question
Even though Amylase can get denatured at high temperatures, a slight increase in temperature, should increase the rate of amylase reaction.
Research title
The effect of temperature on amylase activity.
Word count-1025
Background research
Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all other proteins have secondary and tertiary structures which are affected by temperature, pH and other factors. Due to the fact that enzyme function is related to the structure of any enzyme; enzymes are heavily affected by temperature, pH and other …show more content…
test tube holder
Labels
Marker
Procedure
First take 5 test tubes and label them from 1 to 5.
Label the test plate from 1-5.
Set up 5 water bath to 10 °C .
Add 5 cm3 of starch solution into the 5 labeled test tubes.
Add 5 cm3 of amylase enzyme into other 5 labeled test tubes.
Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C). Do the same for the other test tubes.
Let the test tubes not be disturbed for 3- 4 mins.
Then add the Amylase solution to the Starch solution and start the stopwatch (immediately).
After every 1 min take one drop from the test tubes and place then in the test plate that were labeled.
Add a drop of iodine and mix the solution with a glass rod and the color will begin to change. Observe the color and record them.
This should be continued till the bluish color is almost gone.
The steps should then be repeated for the other 4 temperatures. (for the other 4 test tubes the 5 water baths should be heated to these temperatures 25°C, 35°C, 65°C and room temperature).
Section 2: Data Collection and Processing
2.1 Recording Raw Data
Trial
24°C
35°C
45°C
Even though Amylase can get denatured at high temperatures, a slight increase in temperature, should increase the rate of amylase reaction.
Research title
The effect of temperature on amylase activity.
Word count-1025
Background research
Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all other proteins have secondary and tertiary structures which are affected by temperature, pH and other factors. Due to the fact that enzyme function is related to the structure of any enzyme; enzymes are heavily affected by temperature, pH and other …show more content…
test tube holder
Labels
Marker
Procedure
First take 5 test tubes and label them from 1 to 5.
Label the test plate from 1-5.
Set up 5 water bath to 10 °C .
Add 5 cm3 of starch solution into the 5 labeled test tubes.
Add 5 cm3 of amylase enzyme into other 5 labeled test tubes.
Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C). Do the same for the other test tubes.
Let the test tubes not be disturbed for 3- 4 mins.
Then add the Amylase solution to the Starch solution and start the stopwatch (immediately).
After every 1 min take one drop from the test tubes and place then in the test plate that were labeled.
Add a drop of iodine and mix the solution with a glass rod and the color will begin to change. Observe the color and record them.
This should be continued till the bluish color is almost gone.
The steps should then be repeated for the other 4 temperatures. (for the other 4 test tubes the 5 water baths should be heated to these temperatures 25°C, 35°C, 65°C and room temperature).
Section 2: Data Collection and Processing
2.1 Recording Raw Data
Trial
24°C
35°C
45°C