Temperature On Amylase Essay

Improved Essays
Research question

Even though Amylase can get denatured at high temperatures, a slight increase in temperature, should increase the rate of amylase reaction.

Research title

The effect of temperature on amylase activity.

Word count-1025

Background research

Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all other proteins have secondary and tertiary structures which are affected by temperature, pH and other factors. Due to the fact that enzyme function is related to the structure of any enzyme; enzymes are heavily affected by temperature, pH and other
…show more content…
test tube holder
Labels
Marker

Procedure
First take 5 test tubes and label them from 1 to 5.
Label the test plate from 1-5.
Set up 5 water bath to 10 °C .
Add 5 cm3 of starch solution into the 5 labeled test tubes.
Add 5 cm3 of amylase enzyme into other 5 labeled test tubes.
Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C). Do the same for the other test tubes.
Let the test tubes not be disturbed for 3- 4 mins.
Then add the Amylase solution to the Starch solution and start the stopwatch (immediately).
After every 1 min take one drop from the test tubes and place then in the test plate that were labeled.
Add a drop of iodine and mix the solution with a glass rod and the color will begin to change. Observe the color and record them.
This should be continued till the bluish color is almost gone.
The steps should then be repeated for the other 4 temperatures. (for the other 4 test tubes the 5 water baths should be heated to these temperatures 25°C, 35°C, 65°C and room temperature).

Section 2: Data Collection and Processing
2.1 Recording Raw Data

Trial
24°C
35°C
45°C

Related Documents

  • Improved Essays

    Gel Solution Lab Report

    • 844 Words
    • 4 Pages

    The beaker was stir on the stirring plate until the solution dissolved completely. The already calibrated pH meter was used to measure he initial pH of the buffer as 10.40. HCl was added drop by drop in order to lower the pH to the desired 6.8. A final pH of 6.62 was achieved. Once the buffer was ready, 5 ml of it were added with the graduated cylinder to the initial beaker with the stacking gel.…

    • 844 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    If the solid doesn’t dissolve within 10 minutes. Add another 0.5 g of sodium nitrite. Leave the solution in the ice bath. Part III: Sandmeyer Reaction: Formation of 4-Chlorotoluene Pour the diazonium ion solution (Part 2) into the copper(I) chloride solution (Part 1) and shake the mixture for 5 minutes. Set the system up for a simple distillation.…

    • 2234 Words
    • 9 Pages
    Decent Essays
  • Superior Essays

    Elisa Research Paper

    • 1444 Words
    • 6 Pages

    Incubate the bottles for 5 minutes. Swirl the bottle around to make sure that trypsin removes all the cells from the bottle. Then, use a pipette to remove the substrate from the bottles and into centrifuge tubes. Then centrifuge the tubes for 1 minute at 1000xg. Make sure that the centrifuge tubes are properly balanced.…

    • 1444 Words
    • 6 Pages
    Superior Essays
  • Decent Essays

    to avoid air bubbles Invert the sample bottle 3-4 times immediately prior taking milk for test.  Transfer 10.75ml sample into the butyro meter .  Add 1ml of amyl alcohol and close the neck of butyro meter firmly with the stopper without disturbing the contents. Shake the butyrometer carefully and invert it 2-3 times so that the sample is thoroughly…

    • 2449 Words
    • 10 Pages
    Decent Essays
  • Great Essays

    Beta Vulgaris Experiment

    • 1512 Words
    • 7 Pages

    Pour water into all five water baths and preheat it to temperatures 30, 40,50 ,60 and 70 2. To ensure the water baths are at the right temperature, place a thermometer into all water baths 3. Clear the working area and place a white tile 4. Using a 0.8cm borer, dig out a disc of beetroot and use a ruler to measure 5mm for every beetroot disc. Put the discs on the white tile.…

    • 1512 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    After the initial temperature was recorded the MgO was quickly added and the cup was swirled while the temperature changes were recorded. After the data collection as over the reaction stopped the reaming MgO residue on the watch glass was recorded and all waste products were disposed of and all material were cleaned. The data form reaction four was saved on the lab quest so that reaction five could be carried out. The Styrofoam cups were restacked and placed in a 400 mL beaker to which 120 mL of 2M HCl was then added the calorimeter was then closed and the initial temperature was recorded by using the lab quest. 0.5 g of Mg was then gathered and weighed using the scale and watch glass, all weights were recorded in table 3.…

    • 1835 Words
    • 8 Pages
    Improved Essays
  • Superior Essays

    Enzyme Lab Report

    • 1361 Words
    • 5 Pages

    Use the dropper to drop 1mL of the 10% hydrogen peroxide solution from the third graduated cylinder into the third test tube. Use the stopwatch to record the time for the height of the bubbles to reach maximum height. Observe and record any evidence of a chemical reaction. 14. Repeat steps 2-13 for at least 1 additional trial with three new clean test tubes.…

    • 1361 Words
    • 5 Pages
    Superior Essays
  • Superior Essays

    Amylase Lab

    • 1641 Words
    • 7 Pages

    Each group labels three test tubes with SS and our group names, which ours was 5 dots, and were told to put 4ml of stock starch in each test tub with a pipette. The next step was to get each beaker of 1, 5, and 10 percent concentration of amylase and put 4ml in three other beakers labeled with their respected concentrations. After that was completed our group put all 6 beakers in a water bath that was at 37 degrees Celsius, room temperature, for five minutes. We did that in order for both the substrate and the enzyme to be at its highest effectiveness. While that was going on one of our group members put one drop of IKI, a die that changes the color of starch to a black blue color, in each depression of the spot plate.…

    • 1641 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Starch Investigation

    • 1742 Words
    • 7 Pages

    First, mark 5 test tubes with the temperatures specified above and repeat this for each condition. Using the pipette, insert 5 ml of starch into the labeled test tubes. 2. Then place one test tube into its corresponding water bath and do this for each water bath for a total of 5 test tubes, one in each bath. 3.…

    • 1742 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Next combine 3.5 mL of buffer and 0.5 of your substrate. Then obtain 0.5 sodium carbonate, which was the stop solution. Next add 0.5 mL of enzyme to the buffer and substrate and start the timer. Wait until a pale yellow color appears to add the stop solution and also stop the timer. Then determine the absorbance by using the Spec 20 and recorded the data.…

    • 1525 Words
    • 7 Pages
    Superior Essays