Dna Extraction Lab Report
Table 1: Raw data that shows how changing the mass(g) of soap in the extraction solution affects the mass of DNA extracted from the kiwi (g) Mass of DNA extracted from kiwi (g)
Set Mass of Soap in extraction solution (g) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5
1 0.5 1.25 0.74 0.24 2.04 0.30
2 1 1.41 1.38 1.26 1.52 1.36
3 1.5 1.91 1.48 2.39 2.63 2.00
4 2 2.43 3.34 3.26 3.01 3.62
5 2.5 2.65 4.00 5.32 3.52 3.95
All results are recorded with 2 decimal points for higher accuracy
Numbers highlighted in yellow are outliers
In all of the sets, DNA has formed as a white cloudy mass in the ethanol layer in each test tube.
The DNA is also very fragile and will break apart into loose strands if we agitate it too …show more content…
We also put every set’s kiwi and extraction solution mixture into the water bath together. And in between performing the extraction for each set, the other sets’ was resting and the enzymatic reactions might have started to take place that then destroys the DNA and makes it less intact together after ethanol is added . There are enzymes called DNases found in the cytoplasm and its function is to destroy other DNA’s from virus or sicknesses that infects it. The DNA is normally shielded from the enzyme by the nuclear membrane, but in this experiment, soap has dissolved …show more content…
Have five separate sets of ethanol sitting in the freezer and take out one for use in each set to limit temperature lost and for better setting of DNA. Extending the resting time to ten minutes after adding the ethanol, ensuring that the DNA is fully set before extraction. Set a timer so resting time is accurate. Add 1g of salt instead of 0.5g as it will help the DNA solidify. Preserve the DNA by submerging the beakers in an ice water bath after it has been in the hot water bath to prevent the enzymatic reactions from occurring and destroying the DNA. Transfer and carry the tubes gently and slowly to prevent agitating the DNA too much.
Measuring errors of controlled and independent variables Biased results – directly affects the data collected as it is based on the independent variable. Since 1g of water=1ml, use a digital scale to measure out the water instead of using eye level. To measure out the salt, kiwi and soap, measure accurately to exactly that gram with no extra or less 0.01 grams of that substance. To measure ethanol, use a small syringe for higher