Material and Methods
Restriction enzymes are protein produced by bacteria that cut the DNA at a specific site. For this lab, two restriction enzymes were used to cut the DNA in order to determine which suspects had the potential of committing the crime. In order to conduct the experiment four microtest tubes were used and the tubes were numbered from 1 through 4. The data from table 1 were used to add the correct amount of DNA and enzyme to each microtest tubes (Upadhyaya, 2016). After finishing adding the DNA in each tube, the tubes were added in a water bath for 45 minutes in order to preserve the DNA.
Table 1 Reaction Tube Reaction
Buffer DNA 1
(μL) DNA 2
(μL) Enzyme 1 (μL) Enzyme 2 (μL) Final Volume (μL)
Crime Scene Samples Crime Scene …show more content…
In order to prepare the gel, 50 ml of TBE buffer was obtained and d 0.5 grams of agarose were measure and mixed in a flask. Afterwards, the flask was covered fast to prevent the agarose gel from evaporating. Furthermore, the chemical was heated for one minute; then, it was leave in the table to cool down and reduced the hot temperature. Before pouring the chemical on the tray, a comb was positioned at one end of the tray. After setting the comb, the chemical was then pour on the tray. The gel took about twenty minutes before it could become …show more content…
Similarly, 10 μL of DNA was added from the crime scene with enzyme one and enzyme two in lane 2 and 3. In addition, the DNA of suspect 1 and suspect 2 with enzyme one and enzyme two were added in lane 4 through 7. In total, all 8 lanes were filled with the necessary enzyme. After staining the lane the DNA sample was loaded in the apparatus. The gel was run at 150V and 150mA for 40 minutes in order for it to be electrophoresis (Upadhyaya, 2016). The electricity was able to draw the DNA toward the positive side. When it was time to remove the gel, it was placed under a UV illuminator in order to read the DNA bands the enzyme had made.
Results
The result of the experiment is shown below in table 2. In the solution, suspect one with enzyme 1 matched the crime scene with enzyme 1 (CS 1). However, due to the lack of DNA obtain from suspect one, a blank space is appear in the result although enzyme 2 was added. Nonetheless, suspect two with enzyme 1 matched the crime scene with enzyme 1 just as suspect one with enzyme 1. In addition, suspect two with enzyme two match one base pair (≈3652 bp) while the other base wasn’t present in the result. The other base pair (≈650 bp) in suspect two with enzyme 2 did not match crime scene 2 (CS 2).
Bp base pairs Table
Restriction enzymes are protein produced by bacteria that cut the DNA at a specific site. For this lab, two restriction enzymes were used to cut the DNA in order to determine which suspects had the potential of committing the crime. In order to conduct the experiment four microtest tubes were used and the tubes were numbered from 1 through 4. The data from table 1 were used to add the correct amount of DNA and enzyme to each microtest tubes (Upadhyaya, 2016). After finishing adding the DNA in each tube, the tubes were added in a water bath for 45 minutes in order to preserve the DNA.
Table 1 Reaction Tube Reaction
Buffer DNA 1
(μL) DNA 2
(μL) Enzyme 1 (μL) Enzyme 2 (μL) Final Volume (μL)
Crime Scene Samples Crime Scene …show more content…
In order to prepare the gel, 50 ml of TBE buffer was obtained and d 0.5 grams of agarose were measure and mixed in a flask. Afterwards, the flask was covered fast to prevent the agarose gel from evaporating. Furthermore, the chemical was heated for one minute; then, it was leave in the table to cool down and reduced the hot temperature. Before pouring the chemical on the tray, a comb was positioned at one end of the tray. After setting the comb, the chemical was then pour on the tray. The gel took about twenty minutes before it could become …show more content…
Similarly, 10 μL of DNA was added from the crime scene with enzyme one and enzyme two in lane 2 and 3. In addition, the DNA of suspect 1 and suspect 2 with enzyme one and enzyme two were added in lane 4 through 7. In total, all 8 lanes were filled with the necessary enzyme. After staining the lane the DNA sample was loaded in the apparatus. The gel was run at 150V and 150mA for 40 minutes in order for it to be electrophoresis (Upadhyaya, 2016). The electricity was able to draw the DNA toward the positive side. When it was time to remove the gel, it was placed under a UV illuminator in order to read the DNA bands the enzyme had made.
Results
The result of the experiment is shown below in table 2. In the solution, suspect one with enzyme 1 matched the crime scene with enzyme 1 (CS 1). However, due to the lack of DNA obtain from suspect one, a blank space is appear in the result although enzyme 2 was added. Nonetheless, suspect two with enzyme 1 matched the crime scene with enzyme 1 just as suspect one with enzyme 1. In addition, suspect two with enzyme two match one base pair (≈3652 bp) while the other base wasn’t present in the result. The other base pair (≈650 bp) in suspect two with enzyme 2 did not match crime scene 2 (CS 2).
Bp base pairs Table