1. Where do restriction enzymes come from and what is their original purpose? Restriction enzymes come from bacteria. Bacteria used restriction enzymes to cut up DNA into fragments to fight off viruses. 2. a. Why do you get different fragment sizes if you cut the DNA from two different species with the same restriction enzyme?
Each species has a different arrangement of bases, and the restriction enzyme only cuts after a specific order of bases. Due to that being the case, the specific order of bases won’t be in the same exact spot for each species, so the DNA will be cut in different places. The DNA being cut in different places result with different fragment sizes. b. Why do different restriction enzymes give you different sets …show more content…
If each restriction enzyme cuts at a certain sequence then the fragment sizes would be different because the sequences are spaced out at different intervals.
3. Two men are involved in a paternity suit brought by a woman against her estranged husband. The woman is seeking child support for her infant from her husband, but the husband accused the woman's lover of being the biological father. Unfortunately, blood typing was inconclusive as both men have the same blood type. So the judge ordered DNA analysis. A sample of blood was taken from the mother, the baby, and both men. The results of the gel are shown below.
Who is the father of the baby? Which bands (1-11) are most useful for determining the father of the baby? Why? The lover is the father of the baby. The bands most useful to determine the father of the baby are bands 4 and 8. They are most useful because they are bands that the baby has and only the father (lover) has.
4. Lambda’s is a bacteriophage with a genome 48,502 nucleotides long. The cut sites for the restriction enzyme EcoRI are located at the following nucleotide positions: 21,226 26,104 31,747 39,168 44,972
If EcoRI digest Lambda DNA, determine the number of fragments and calculate the lengths of each
Each species has a different arrangement of bases, and the restriction enzyme only cuts after a specific order of bases. Due to that being the case, the specific order of bases won’t be in the same exact spot for each species, so the DNA will be cut in different places. The DNA being cut in different places result with different fragment sizes. b. Why do different restriction enzymes give you different sets …show more content…
If each restriction enzyme cuts at a certain sequence then the fragment sizes would be different because the sequences are spaced out at different intervals.
3. Two men are involved in a paternity suit brought by a woman against her estranged husband. The woman is seeking child support for her infant from her husband, but the husband accused the woman's lover of being the biological father. Unfortunately, blood typing was inconclusive as both men have the same blood type. So the judge ordered DNA analysis. A sample of blood was taken from the mother, the baby, and both men. The results of the gel are shown below.
Who is the father of the baby? Which bands (1-11) are most useful for determining the father of the baby? Why? The lover is the father of the baby. The bands most useful to determine the father of the baby are bands 4 and 8. They are most useful because they are bands that the baby has and only the father (lover) has.
4. Lambda’s is a bacteriophage with a genome 48,502 nucleotides long. The cut sites for the restriction enzyme EcoRI are located at the following nucleotide positions: 21,226 26,104 31,747 39,168 44,972
If EcoRI digest Lambda DNA, determine the number of fragments and calculate the lengths of each